|Table of Contents|

Cloning and Bioinformatics Analysis of LhrMYB1,LhrMYB12,LhrMYB15 and LhrMYB16 Genes in Lilium henryi Baker

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年12
Page:
42-51
Research Field:
Publishing date:

Info

Title:
Cloning and Bioinformatics Analysis of LhrMYB1,LhrMYB12,LhrMYB15 and LhrMYB16 Genes in Lilium henryi Baker
Author(s):
DONG Ran1JIN Ge23LIN Qiang23HAN Dongyang23ZHONG Huiye34ZHANG Xiuhai3
(1.Liupao Tea Modern Industry College,Wuzhou University,Wuzhou,Guangxi 543002;2.College of Forestry and Grassland Science,Jilin Agricultural University,Changchun,Jilin 130118;3.Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097;4.College of Resources and Environment,Xizang Agriculture and Animal Husbandry College,Nyingchi,Xizang 860000)
Keywords:
Lilium henryi BakerMYBgene cloningbioinformatics analysis
PACS:
S 682.29
DOI:
10.11937/bfyy.20234392
Abstract:
Taking Lilium henryi Baker as the test material,the MYB gene was cloned using RT-PCR method.The structure and function of the protein encoded by the MYB gene were studied using tools such as DNAMAN 8.0,ExPaSy-ProParam,and NetPhos 3.1 Server,in order to provide reference for the study of MYB transcription factors relused to Lilium henryi Baker.The results showed that LhrMYB1 and LhrMYB15 proteins were stable proteins,alkaline proteins,and hydrophilic proteins,while LhrMYB12 protein was stable protein,acidic protein,and hydrophilic protein;LhrMYB16 protein was an unstable protein,alkaline protein,and hydrophilic protein.LhrMYB1 had the closest relationship with MYB3-like in mango (XM 044620835),LhrMYB12 had the closest relationship with lily hybrid variety (MK182389), LhrMYB15 had the closest relationship with lily hybrid variety division I MYB15like (LC218141),and LhrMYB16 had the closest relationship with lily hybrid variety division I MYB16-1 (LC218139).

References:

[1]夏婷,耿兴敏,罗凤霞.不同花色野生百合色素成分分析[J].东北林业大学学报,2013,41(5):109-113,166.[2]GROTEWOLD E.The genetics and biochemistry of floral pigments[J].Annual Review of Plant Biology,2006,57:761-780.[3]ZHENG T,LI P,LI L,et al.Research advances in and prospects of ornamental plant genomics[J].Horticulture Research,2021(8):65.[4]YAMAGISHI M.How genes paint lily flowers:Regulation of colouration and pigmentation patterning[J].Scientia Horticulturae,2013,163:27-36.[5]吕波,张文政,李春雨,等.MYB家族转录因子OsMYB84通过ABA信号通路参与盐胁迫响应[J].复旦学报(自然科学版),2015,54(5):591-600.[6]ISHIDA T,KURATA T,OKADA K,et al.A genetic regulatory network in the development of trichomes and root hairs[J].Annual Review of Plant Biology,2008,59:365-386.[7]NADEAU J A.Stomatal development:New signals and fate determinants[J].Current Opinion in Plant Biology,2009,12(1):29-35.[8]WANG J,LIU Y,TANG B,et al.Genome-wide identification and capsaicinoid biosynthesis-related expression analysis of the R2R3-MYB gene family in Capsicum annuum L[J].Frontiers in Genetics,2020(11):598183.[9]牛义岭,姜秀明,许向阳.植物转录因子MYB基因家族的研究进展[J].分子植物育种,2016,14(8):2050-2059.[10]GROTEWOLD E,SAINZ M B,TAGLIANI L,et al.Identification of the residues in the Myb domain of maize C1 that specify the interaction with the bHLH cofactor R[J].Proceedings of the National Academy of Sciences of the United States of America,2000,97(25):13579-13584.[11]张群华,方玉占,杜建科,等.园艺植物R2R3-MYB转录因子研究现状[J].分子植物育种,2024,22(1):85-96.[12]LIPSICK J S.One billion years of MYB[J].Oncogene,1996,13(2):223-235.[13]朱珏,石雨荷,李晴,等.百合种质资源遗传多样性及连作障碍研究进展[J].北方园艺,2023(9):126-132.[14]胡佳诺,余梓萌,张心怡,等.莲的花色研究进展[J].植物科学学报,2023,41(6):800-808.[15]RUDALL P J.Colourful cones:How did flower colour first evolve?[J].Journal of Experimental Botany,2020,71(3):759-767.[16]MEKAPOGU M,VASAMSETTI B M K,KWON O K,et al.Anthocyanins in floral colors:Biosynthesis and regulation in Chrysanthemum flowers[J].International Journal of Molecular Sciences,2020,21(18):6537.[17]WANG X C,WU J,GUAN M L,et al.Arabidopsis MYB4 plays dual roles in flavonoid biosynthesis[J].The Plant Journal:for Cell and Molecular Biology,2020,101(3):637-652.[18]LIU X,FENG C,ZHANG M,et al.The 〖STBX〗MrWD40-1 〖STBZ〗gene of Chinese bayberry (Myrica rubra) interacts with MYB and bHLH to enhance anthocyanin accumulation[J].Plant Molecular Biology Reporter,2013,31(6):1474-1484.[19]GAO Y,LIU J,CHEN Y,et al.Tomato SlAN11 regulates flavonoid biosynthesis and seed dormancy by interaction with bHLH proteins but not with MYB proteins[J].Horticulture Research,2018,5:27.[20]KOES R,VERWEIJ W,QUATTROCCHIO F.Flavonoids:A colorful model for the regulation and evolution of biochemical pathways[J].Trends in Plant Science,2005,10(5):236-242.[21]FELLER A,MACHEMER K,BRAUN E L,et al.Evolutionary and comparative analysis of MYB and bHLH plant transcription factors[J].The Plant Journal:for Cell and Molecular Biology,2011,66(1):94-116.[22]LAI Y,LI H,YAMAGISHI M.A review of target gene specificity of flavonoid R2R3-MYB transcription factors and a discussion of factors contributing to the target gene selectivity[J].Frontiers in Biology,2013,8(6):577-598.[23]YAMAGISHI M.Oriental hybrid lily Sorbonne homologue of 〖STBX〗LhMYB12 〖STBZ〗regulates anthocyanin biosyntheses in flower tepals and tepal spots[J].Molecular Breeding,2011,28(3):381-389.[24]SAITOH K,ONISHI K,MIKAMI I,et al.Allelic diversification at the C (OsC1) locus of wild and cultivated rice:Nucleotide changes associated with phenotypes[J].Genetics,2004,168(2):997-1007.

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Last Update: 2024-07-11