|Table of Contents|

Tissue Culture and Establishment of Regeneration System of Astragalus membranaceus

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年14
Page:
109-115
Research Field:
Publishing date:

Info

Title:
Tissue Culture and Establishment of Regeneration System of Astragalus membranaceus
Author(s):
LI Jingjing; RAN Pan; WANG Jia; ZHU Qiang; WANG Lingxia; LIANG Wenyu
(College of Life Sciences,Ningxia University,Yinchuan,Ningxia 750021)
Keywords:
Astragalus membranaceus; tissue culture; regenerated plants; germplasm resources
PACS:
S 326
DOI:
10.11937/bfyy.20234354
Abstract:
Taking the young leaves,stems,and hypocotyls of Astragalus membranaceus as the test materials,different concentrations and combinations of 6-BA,NAA,IAA,IBA,and TDZ were added to MS medium to study the establishment of callus and adventitious bud induction differentiation and regeneration systems of Astragalus membranaceus.The aim was to construct a complete system for tissue culture and plant regeneration of Astragalus membranaceus,in order to provide reference for the protection and utilization of Astragalus membranaceus resources,and laid a technical foundation for the further rapid propagation of seedlings and genetic improvement of Astragalus membranaceus.The results showed that young stems were the best explants for callus,and the best medium for callus induction was MS+6-BA 1.0 mg·L-1+NAA 0.5 mg·L-1+TDZ 0.2 mg·L-1,and the induction rate was 90.00%.The optimal medium for inducing adventitious buds from callus was MS+6-BA 1.0 mg·L-1+NAA 0.3 mg·L-1,and the induction rate was 76.60%.The optimal medium for inducing adventive buds from young stems was MS+6-BA 1.5 mg·L-1+IAA 0.2 mg·L-1,and the induction rate was 90.30%.The optimal medium for rooting culture was 1/2MS+IBA 0.5 mg·L-1+IAA 1.0 mg·L-1,and the rooting rate was 39.30%.

References:

［1］冯学金，刘根科，梁素明．蒙古黄芪种质资源研究进展［J］．山西农业科学，2010，38(8)：95-98.［2］郭生虎，陈虞超，李明，等．膜荚黄芪愈伤组织诱导及植株再生体系的建立［J］．分子植物育种，2020，18(18)：6122-6126.［3］吴师竹，贺正全．黄芪类品种的药理研究［J］．中药药理与临床，1992，8(6)：24-27.［4］马斌，李明，李海洋，等．双层覆膜对土壤特性及黄芪、黄芩生长的影响［J］．北方园艺，2023(19)：92-99.［5］王强雄，郭盛，李会伟，等．不同产地蒙古黄芪茎叶UPLC指纹图谱建立及化学模式识别研究［J］．中草药，2023，54(13)：4312-4320.［6］田雨，高新新，张秀娟，等．蒙古黄芪与膜荚黄芪愈伤组织诱导及再生植株体系的建立［J］．分子植物育种，2022，20(15)：5080-5087.［7］赖士淦，巫智斌，陈剑勇，等．福建青冈组织培养过程中的外植体褐变机理与调控技术［J］．林业科技通讯，2023(8)：75-78.［8］姚宁,马晓蓉,高建中,等.银柴胡组织培养与植株再生体系的建立［J/OL］.分子植物育种，(2021-09-22)［2024-04-09］.http://kns.cnki.net/kcms/detail/46.1068.S.20210920.0014.002.html.［9］戴莹，杨世海，赵鸿峥，等．药用植物组织培养中褐化现象的研究进展［J］．中草药，2016，47(2)：344-351.［10］胡海英，吴晓玲．膜荚黄芪愈伤组织诱导培养［J］．江苏农业科学，2005，33(2)：97-98.［11］WANG C，MA H，ZHU W，et al．Seedling-derived leaf and root tip as alternative explants for callus induction and plant regeneration in maize［J］．Physiologia Plantarum，2021，172(3)：1570-1581.［12］CHIRVA O V，IGNATENKO R V，ERSHOVA M A．Pinus sylvestris L．mature seeds megagamethophytes cultured in vitro.Influence of the genotype and the growth regulators content in nutrient medium on the initiation of callus formation［J］．Plant Cell，Tissue and Organ Culture (PCTOC)，2023，152(2)：299-308.［13］JIMNEZ V M，BANGERTH F．Hormonal status of maize initial explants and of the embryogenic and non-embryogenic callus cultures derived from them as related to morphogenesis in vitro［J］．Plant Science：An International Journal of Experimental Plant Biology，2001，160(2)：247-257.［14］EL ABIDINE TRIQUI Z，GUDIRA A，CHLYAH A，et al．Effect of genotype，gelling agent，and auxin on the induction of somatic embryogenesis in sweet potato (Ipomoea batatas Lam．)［J］．Comptes Rendus Biologies，2008，331(3)：198-205.［15］MARGARITA P,CANTERO-NAVARRO E,FRANCISCO P,et al.Relationship between endogenous hormonal content and somatic organogenesis in callus of peach (Prunus persica L.Batsch) cultivars and Prunus persica×Prunus dulcis rootstocks［J］.Journal of Plant Physiology,2014,171(8):619-624.［16］SANDHU R，KENNEDY J，ROBERT N，et al.Plant development and biotechnology［J］．Carbohydrate Polymers，2006，63(4)：561-562.［17］DALILA Z D，JAAFAR H，MANAF A A．Effects of 2，4-D and kinetin on callus induction of Barringtonia racemosa leaf and endosperm explants in different types of basal media［J］．Asian Journal of Plant Sciences，2012，12(1)：21-27.

Memo

Memo:
-
Last Update: 2024-08-08