|Table of Contents|

Polyploid Induction and Identification of Carex breviculmis ‘Siji’

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年15
Page:
52-59
Research Field:
Publishing date:

Info

Title:
Polyploid Induction and Identification of Carex breviculmis ‘Siji’
Author(s):
JIA Ming1; 2; DONG Shuanghui1; CHAO Luomeng2; FAN Xifeng1; WU Juying1; YUE Yuesen1
(1.Institute of Grassland,Flowers and Landscape Ecology,Beijing Academy of Agriculture and Forestry Sciences/North China Key Laboratory of Urban Agriculture,Ministry of Agriculture and Rural Affairs,Beijing 100097；2.School of Ecology and Environment,Inner Mongolia University,Hohhot,Inner Mongolia 010021)
Keywords:
C.breviculmis ‘Siji’; polyploidy induction; colchicine; ploidy identification
PACS:
S 688.4
DOI:
10.11937/bfyy.20234336
Abstract:
Taking the embryogenic callus of Carex breviculmis ‘Siji’ as the test material,selecting colchicine as the inducer,and using the colchicine soaking method for mutagenesis treatment.The optimal conditions for inducing polyploidy of Carex breviculmis ‘Siji’ were under different colchicine concentrations and different treatments times.The ploidy of doubled plants was identified by flow cytometry and root tip chromosome counting,and leaf stomatal characteristics and plant phenotypic characteristics were analyzed,in order to provide reference for the polyploid induction of Carex breviculmis ‘Siji’ and the cultivation of new Carex varieties.The results showed that the induction rate was the highest when the colchicine concentration was 0.10% and the treatment time was 48 hours,and the induction rate under these conditions could reach 65%.Comparing the plant phenotype and stomatal characteristics,it was found that the doubled plants were significantly different from the diploid plants,which were characterized by broad leaf shape,reduced plant height,and enlarged stomatal pores.

References:

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Last Update: 2024-08-23