|Table of Contents|

Establishment of a Rapid Propagation System for in vitro Stem Segments of Lagerstroemia indica ‘Wuye’

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年12
Page:
52-58
Research Field:
Publishing date:

Info

Title:
Establishment of a Rapid Propagation System for in vitro Stem Segments of Lagerstroemia indica ‘Wuye’
Author(s):
MA Qingqing1; 2; 3; JIAO Ziwei1; 2; 3; SHEN Hongming4; WANG Guolian4; ZHAO Yu1; 2; 3; GU Cuihua1; 2; 3
(1.College of Landscape and Architecture，Zhejiang A&F University，Hangzhou，Zhejiang；2.Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants，Zhejiang A&F University，Hangzhou，Zhejiang；3.Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants，Zhejiang A&F University，Hangzhou，Zhejiang；4.Zhejiang Sencheng Seed Industry Limited Company，Haining，Zhejiang)
Keywords:
Lagerstroemia indica ‘Wuye’; stem segments; axillary bud induction; proliferation; rooting
PACS:
S 723.1
DOI:
10.11937/bfyy.20233914
Abstract:
Taking the young stem segments with axillary buds of a new variety of Lagerstroemia indica ‘Wuye’ as explants，the orthogonal experimental method was used to study the effects of disinfection time of different combinations of NaClO and C2H5OH on the contamination and germination of the explants.The effects of three basic media，WPM，1/2MS，and MS，as well as different concentration combinations of 6-BA and NAA，on the induction and proliferation of axillary buds from tender stems of L.indica ‘Wuye’ were studied，as well as the effects of three basic media and different concentration combinations of IBA on the rooting of L.indica ‘Wuye’ were studied，in order to provide reference for mass propagation of Lagerstroemia indica ‘Wuye’.The results showed that,when the disinfection time of 75% C2H5OH was 30 seconds and the disinfection time of 5% NaClO was 6 minutes，the pollution rate was lower，at 18.6%.The WPM medium was suitable for the induction and proliferation of axillary buds in stem segments of L.indica ‘Wuye’.The highest induction rate was achieved when the concentrations of 6-BA and NAA were 1.0 mg·L-1 and 0.5 mg·L-1，respectively，reaching 93.28%.When the concentration of 6-BA was 1.0 mg·L-1 and the concentration of NAA was 0.1 mg·L-1，the highest proliferation coefficient was 5.83.The concentration change of IBA had a significant impact on the rooting of L.indica ‘Wuye’.Adding 0.5 mg·L-1 IBA to the 1/2MS had the highest rooting rate of 92.99%，and the growth of sterile seedlings was good.After refining the seedlings，transplanted the tissue culture seedlings onto a substrate of peat soil∶perlite∶vermiculite in a ratio of 3∶1∶2，with a survival rate of up to 91%.

References:

［1］方文培.中国植物志：第52卷第2分册［M］.北京：科学出版社，1983.［2］刘芙蓉,胡娉婷,罗建勋,等.紫薇新品种‘蜀紫粉’［J］.园艺学报,2021,48(1):201-202.［3］秦波,冯露,王佳,等.紫薇新品种‘玲珑’［J］.园艺学报，2020，47(增刊2)：3113-3114.［4］蔡能,乔中全,王晓明,等.紫薇新品种‘彩霞’［J］.园艺学报，2020，47(2)：403-404.［5］ZHANG Q Y,LUO F X,LIU L,et al.In vitro induction of tetraploids in crape myrtle(Lagerstroemia indica L.)［J］.Plant Cell Tissue and Organ Culture,2010,101(1):41-47.［6］EGOLF D R.‘Choctaw’ Lagerstroemia［J］.Hortscience,1990,25(8):992-993.［7］EGOLF D R.‘Muskogee’ and ‘Natchez’ Lagerstroemia［J］.Hortscience,1981,16(4):576-577.［8］EGOLF D R.‘Tuskegee’ Lagerstroemia［J］.Hortscience,1981,16(6):788-789.［9］王莹,李敏,谈峰,等.紫叶紫薇新品系叶色变化理化因素研究［J］.江苏农业科学,2018,46(24):150-153.［10］李永欣,王晓明,乔中全,等.紫叶紫薇引种试验［J］.湖南林业科技,2018,45(6):68-71.［11］王晓明,李永欣,曾慧杰,等.‘红叶’等美国紫薇新品种品比试验［J］.湖南林业科技,2018,45(6):9-13.［12］陈卓梅,沈鸿明,张冬林,等.从美国专利品种看中国紫薇育种发展趋势［J］.浙江林业科技,2018,38(1):49-61.［13］饶丹丹,王湘莹,蔡能,等.紫叶紫薇良种组培快繁研究［J］.中南林业科技大学学报,2020,40(12):75-82.［14］黄钦才.紫薇腋芽培养［J］.植物生理学通讯,1984,20(3):44.［15］JOHN P.In vitro clonal propagation of Lagerstroemia flos-reginae Retz［J］.Plant Cell Tissue and Organ Culture,1986,6(1):41-45.［16］姜旭红,宋刚,张虎,等.日本紫薇的组织培养与快速繁殖［J］.植物生理学通讯,2004,40(6):707.［17］段丽君,李国瑞,童俊,等.紫薇离体茎段快速繁殖体系研究［J］.江西农业大学学报,2013,35(4):709-714.［18］陈怡佳,崔媛媛,张晓明,等.美国红叶紫薇的组织培养与快速繁殖［J］.植物生理学报,2015,51(6):882-886.［19］鲁好君,崔媛媛,白红娟,等.不同激素处理对红火球紫薇组织培养的影响［J］.广东林业科技,2015,31(6):52-56.［20］吕铭,王肖雄,何汝峰,等.红叶紫薇“一步法”快速繁殖试验［J］.江苏林业科技,2017,44(3):15-18,22.［21］陈晓航.美国黑钻紫薇组培快繁体系的建立［D］.南京:南京农业大学,2017.［22］黄菲颖.‘紫精灵’紫薇组织培养及内源激素含量变化研究［D］.长沙:中南林业科技大学,2022.［23］张晴，王湘莹，陈亮明，等.‘紫琦’紫薇组织培养及增殖过程中内源激素含量变化［J］.中南林业科技大学学报，2023，43(7)：101-108.［24］张新超,张新宇,张雪梅，等.‘绿岭’核桃带芽茎段的组织培养和快速繁殖［J］.北方园艺,2023(24):38-44.［25］MOHAMMAD N.Tissue culture and its modern usage［J］.Cell & Developmental Biology,2021,10(2):1.［26］梁建,刘世晗,刘桢，等.紫薇属植物组织培养技术研究进展［J］.北方园艺,2021(23):142-149.［27］罗嘉亮,李凡,郝瑞杰,等.外源生长素对杜梨组培不定芽生根及相关氧化酶的影响［J］.经济林研究,2020,38(1):125-132.

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Last Update: 2024-07-11