|Table of Contents|

Effects of Nitrate Nitrogen on the Growth and Heterophyllin B of Pseudostellaria heterophylla

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年7
Page:
104-111
Research Field:
Publishing date:

Info

Title:
Effects of Nitrate Nitrogen on the Growth and Heterophyllin B of Pseudostellaria heterophylla
Author(s):
LIU Yang; OU Xiaohong; HU Yipu; XU Jiao; HE Hua; ZHOU Tao
(Resource Institute for Chinese and Ethnic Materia Medica,Guizhou University of Traditional Chinese Medicine，Guiyang，Guizhou 550025)
Keywords:
P.heterophylla; nitrate nitrogen; growth and development; nutrient content; heterophyllin B
PACS:
S 567.53
DOI:
10.11937/bfyy.20233616
Abstract:
The variety ‘Shitai No.1’ of Pseudostellaria heterophylla was used for experimental material.Different nitrate usages were designed to study the growth and development，as well as heterophyllin B content of P.heterophylla by tissue culture method,in order to provide reference for the nitrogen dosage in the cultivation of P.heterophylla tissue culture seedlings.The results showed that the plants of P.heterophylla with no nitrogen addition had a phenotype of short plant，leaves chlorosis，less root formation and enlongation;while with nitrate addition as N resource，the plant could grow normally with higher plant height，more leaves with dark green，more roots formation.Compared with no N addition，supplying nitrate significantly increased the activities of antioxidant enzymes and N metabolism enzymes，enhanced the mineral nutrients uptake.The best growth of P.heterophylla was achieved at supplying 20 mmol·L-1 nitrate;the highest heterophyllin B content and prePhHB expression of P.heterophylla，which were positively correlated with the N content，were both gained at supplying 10 mmol·L-1 nitrate.This means improving N content of P.heterophylla could increase the accumulation of heterophyllin B.In conclusion，supplying 10-20 mmol·L-1 nitrate as the N resource in tissue culture of P.heterophylla，could promote the growth and development as well as the accumulation of heterophyllin B.

References:

［1］国家药典委员会.中华人民共和国药典:一部［M］.北京:中国医药科技出版社,2020.［2］TANTAI J C,ZHANG Y,ZHAO H.Heterophyllin B inhibits the adhesion and invasion of ECA109 huma-n esophageal carcinoma cells by targeting PI3K/AKT/βcatenin signaling［J］.Molecular Medicine Reports,2016,13(2):1097-1104.［3］吴红淼,张晟恺,焦艳阳,等.微生物菌肥对太子参连作障碍和药理作用的改良效应［J］.中国生态农业学报(中英文),2021,29(8):1315-1326.［4］李玺洋,苏桢,申火云,等.太子参绿色种植模式田间试验效果［J］.中国测试,2022,48(S1):50-56.［5］赵坤,李一平,卫臻,等.小麦生长季土壤不同形态氮素的含量及淋失特征［J］.节水灌溉,2013(11):12-16.［6］LEE E J,PAEK K Y.Effect of nitrogen source on biomass and bioactive compound production in submerged cultures of Eleutherococcus koreanum Nakai adventitious roots［J］.Biotechnology Progress,2012,28(2):508-514.［7］WANG Y Y,CHENG Y H,CHEN K E,et al.Nitrate transport,signaling,and use efficiency［J］.Annual Review of Plant Biology,2018,69:85-122.［8］李灿雯,王康才,吴健,等.氮素形态对半夏生长及生物碱和总有机酸累积的影响［J］.植物营养与肥料学报,2012,18(1):256-260.［9］巩金壮,焉学倩,陈艳阳,等.氮素形态对人参氮代谢相关酶活性的影响［J］.吉林农业大学学报,2023,45(6):709-715.［10］朱波,周明华,况福虹,等.紫色土坡耕地氮素淋失通量的实测与模拟［J］.中国生态农业学报,2013,21(1):102-109.［11］罗江宏,李杰,李娟等.施氮水平和间断施氮对生菜生长和品质的影响［J］.北方园艺,2022(11):23-30.［12］隋利,易家宁,王康才,等.不同氮素形态及其配比对盐胁迫下紫苏生理特性的影响［J］.生态学杂志,2018,37(11):3277-3283.［13］张成才,王升,王月枫,等.药用植物组织培养技术在中药资源可持续发展中的应用研究［J］.中国中药杂志,2023,48(5):1186-1193.［14］LI B,LI Q,SU Y,et al.Shoot-supplied ammonium targets the root auxin influx carrier AUX1 and inhibits lateral root emergence in Arabidopsis［J］.Plant,Cell & Environment,2011,34(6):933-946.［15］李合生.植物生理生化实验原理和技术［M］.北京:高等教育出版社,2000.［16］毕艳.基于太子参环肽差异种质ST1和ZS2分析环肽HB的合成调控机制［D］.贵阳:贵州中医药大学,2021.［17］崔佩佩,丁玉川,焦晓燕,等.氮肥对作物的影响研究进展［J］.山西农业科学,2017,45(4):663-668.［18］张莉,马国珠,王婧等.不同氮素形态对茅苍术生长和氮磷钾吸收的影响［J］.北方园艺,2022(15):106-112.［19］郭芸珲,于媛媛,温立柱,等.硝态氮影响菊花根系形态结构变化的分子基础［J］.中国农业科学,2017,50(9):1684-1693.［20］王莎莎,段文学,张海燕,等.硝态氮对不同耐氮性甘薯苗期根系生长和氮代谢的影响［J］.山东农业科学,2022,54(1):55-60.［21］赵姣姣,刘文科,杨其长.氮素水平和形态对基质栽培桔梗生长及生理参数的影响［J］.北方园艺,2013(4):162-165.［22］王媚,宋建平,韩乐,等.太子参环肽B含量分析及其动态研究［J］.中药材,2010,33(8):1225-1227.［23］肖承鸿,周涛,江维克,等.栽培太子参表型性状的遗传多样性分析［J］.中药材,2015,38(8):1600-1606.［24］肖云华,赵雪玲,王康才,等.不同氮素形态和浓度对大青叶生物量与生物碱类成分的影响［J］.中国中药杂志,2013,38(17):2755-2760.

Memo

Memo:
-
Last Update: 2024-05-10