|Table of Contents|

Functional Analysis of Phospholipase PcPLD8 Gene From Phytophthora capsici

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年17
Page:
1-7
Research Field:
Publishing date:

Info

Title:
Functional Analysis of Phospholipase PcPLD8 Gene From Phytophthora capsici
Author(s):
YANG Lei1; 2; ZHU Tongtong1; 3; LIU Yingbao1; SUN Wenxiu1
Keywords:
Phytophthora capsici; phospholipase D; pathogenicity; functional analysis
PACS:
S 436.418
DOI:
10.11937/bfyy.20230315
Abstract:
Phytophthora capsici was used as test material,the function of phospholipase D gene was studied by gene silencing and gene overexpression,the effects of gene on the growth and pathogenicity in P.capsici were revealed,in order to provide reference for further revealing the mechanism of phospholipase D in the pathogenesis of P.capsici. The results showed that the silenced transformants S1 and S5 of gene had sparse hyphae,many and short lateral branches,small colony diameter and slow growth rate,the pathogenicity to Nicotiana benthamiana decreased obviously,compared with the wild-type strain WT and the empty vector transformant CK. And over-expressed transformant OE2 showed little change in hypha morphology,growth rate and pathogenicity to N.benthamiana.

References:

［1］ 李春霞,吴兴彪,贺翰林,等.氮肥配施复硝酚钠对辣椒不同生育期果实品质的影响［J］.北方园艺,2022(13):1-7.［2］ FAN G,ZOU A,WANG X,et al.Polymorphic microsatellite development,genetic diversity,population differentiation and sexual state of Phytophthora capsici on commercial peppers in three provinces of southwest China［J］.Applied and Environmental Microbiology,2022,88(23):e0161122.［3］ LAMOUR K H,STAM R,JUPE J,et al.The oomycete broad-host-range pathogen Phytophthora capsici［J］.Molecular Plant Pathology,2012,13(4):329-337.［4］ OELKE L M,BOSLAND P,STEINER R.Differentiation of race specific resistance to Phytophthora root rot and foliar blight in Capsicum annuum［J］．Journal of the American Society for Horticultural Science,2003,128:213-218.［5］ 席亚东,陈国华,谢丙炎,等.辣椒疫霉菌全球传播与危害及生物学特性研究进展［J］.北方园艺,2016(11):199-203.［6］ BARGMANN B O,MUNNIK T.The role of phospholipase D in plant stress responses［J］.Curr Opin Plant Biol,2006,9(5):515-522.［7］ ZHANG W,YU L,ZHANG Y,et al.Phospholipase D in the signaling networks of plant response to abscisic acid and reactive oxygen species［J］.Biochim Biophys Acta,2005,1736(1):1-9.［8］ LI M,HONG Y,WANG X.Phospholipase D-and phosphatidic acid-mediated signaling in plants［J］.Biochimicaet Biophysica Acta,2009,1791(9):927-935.［9］ LI J,WANG X.Phospholipase D and phosphatidic acid in plant immunity［J］.Plant Sci,2019,279:45-50.［10］BARMAN A,GOHAIN D,BORA U,et al.Phospholipases play multiple cellular roles including growth,stress tolerance,sexual development,and virulence in fungi［J］.Microbiol Res,2018,209:55-69.［11］MENDONSA R,ENGEBRECHT,J.Phospholipase D function in Saccharomyces cerevisiae［J］.Biochimica Et Biophysica Acta,2009,1791:970-974.［12］NAKANISHI H,MORISHITA M,SCHWARTZ C L,et al.Phospholipase D and the SNARE Sso1p are necessary for vesicle fusion during sporulation in yeast［J］.Journal of Cell Science,2006,119(7):1406-1415.［13］RIEDEL C G,MAZZA M,MAIER P,et al.Differential requirement for phospholipase D/Spo14 and its novel interactor Sma1 for regulation of exocytotic vesicle fusion in yeast meiosis［J］.J Biol Chem,2005,280(45):37846-37852.［14］YANG F,ABDELNABBY H,XIAO Y.The role of a phospholipase (PLD) in virulence of Purpureocillium lilacinum (Paecilomyces lilacinum)［J］.Microbial Pathogenesis,2015,85:11-20.［15］DING M,ZHU Q,LIANG Y,et al.Differential roles of three FgPLD genes in regulating development and pathogenicity in Fusarium graminearum［J］.Fungal Genet Biol,2017,109:46-52.［16］MEIJER H J G,SCHOINA C,WANG S,et al. Phytophthora infestans small phospholipase D-like proteins elicit plant cell death and promote virulence［J］.Mol Plant Pathol,2019,20(2):180-193.［17］杨磊,朱彤彤,刘应保,等.辣椒疫霉菌PcPLD8基因的克隆和表达分析［J］.湖南师范大学自然科学学报,2021,44(1):91-96.［18］ENKERLI K M C W，HAHN M G.Ultrastructure of compatible and incompatible interactions of soybean roots infected with the plant pathogenic oomycete Phytophthora sojae［J］.Canadian Journal of Botany,1997,75(9):1493-1508.［19］ZHU T T,XIANG S H,YANG L,et al.Characterization,expression patterns and functional analysis of PcMPK12 gene in Phytophthora capsica［J］.Europe Journal of Plant Pathology,2022,164:525-537.［20］KHRONGYUT S,PAKKARATO S,WATTHANAKITPHIBUN A,et al.Expression with early postnatal peak and female-dominant sexual dimorphism of phospholipase D (PLD) 2 in submandibular gland ducts in situ of mice［J］.Odontology,2023,111(3):565-572.［21］WU Z L,XU H Q,WANG P,et al.The entry of unclosed autophagosomes into vacuoles and its physiological relevance［J］.PLoS Genetics,2022,39:401-411. ［22］WANG N Y,LIU C H,CHUNG K R.A Gα subunit gene is essential for conidiation and potassium efflux but dispensable for pathogenicity of Alternaria alternata on citrus［J］.Curr Genet,2010,56:43-51.［23］LI Y,QUE Y W,LIU Y T,et al.The putative Gγ subunit gene MGG1[STBZ] is required for conidiation,appressorium formation,mating and pathogenicity in Magnaporthe oryzae［J］.Curr Genet,2015,61:641-651.［24］LIANG X,WEI T,CAO M,et al.The MAP kinase CfPMK1 is a key regulator of pathogenesis,development,and stress tolerance of Colletotrichum fructicola［J］.Frontiers in Microbiology,2019,1(10):1070.［25］GAO J,CAO M,YE W,et al.PsMPK7,a stress-associated mitogen-activated protein kinase (MAPK) in Phytophthora sojae,is required for stress tolerance,reactive oxygenated species detoxification,cyst germination,sexual reproduction and infection of soybean［J］.Molecular Plant Pathology,2015,16(1):61-70.

Memo

Memo:
-
Last Update: 2023-10-13