|Table of Contents|

Differential Analysis of MYB Family Members of Transcription Factors in the Leaves of Two Different Varieties of Honeysuckle

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2026年5
Page:
29-38
Research Field:
Publishing date:

Info

Title:
Differential Analysis of MYB Family Members of Transcription Factors in the Leaves of Two Different Varieties of Honeysuckle
Author(s):
ZUO Hongjuan1; ZHANG Zhenhua2; ZHANG Xiaoshen1; CAO Hui1; CHEN Yue2; NIU Suyan2
(1.Zhengzhou Academy of Agricultural Science and Technology，Zhengzhou，Henan 450005；2.Zhengzhou Normal University,Zhengzhou，Henan 450044)
Keywords:
Lonicera japonica; MYB transcription factor; transcriptome sequencing; bioinformatics analysis
PACS:
S661.2
DOI:
10.11937/bfyy.20252855
Abstract:
Taking leaves of the honeysuckle new varieties ‘Zhengyinhua No.3’ and ‘Fenghua No.1’ as the test materials，bioinformatics methods were employed to analyze the physicochemical properties，secondary structure，motif comparison，phylogenetic relationships，and functional annotations of MYB transcription factors in honeysuckle. The study examined the expression differences of MYB transcription factors in the leaves of these two honeysuckle varieties,in order to provide reference for exploration of the differences and functional characterization of MYB family members in the leaves of these two honeysuckle varieties.The results showed that there were 52 members of the MYB transcript factor family in the leaves of two varieties，mainly distributed on chromosomes 5 and 9.All proteins showed hydrophilicity，with 23 proteins being acidic and 29 proteins being alkaline.The motif analysis showed that 49 transcription factors included Motif 3，which had the highest frequency of occurrence，while all 34 transcription factors contained Motif 3，Motif 2，and Motif 1.The heat map indicated that there were 14 genes with significant differences in expression levels between the two varieties.Combined with GO functional annotation，it was found that these 14 differentially expressed genes might be related to the growth and development in Lonicera japonica.

References:

［1］PYSH L D,WYSOCKA-DILLER J W,CAMILLERI C,et al.The GRAS gene family in Arabidopsis:Sequence characterization and basic expression analysis of the SCARECROW-LIKE genes［J］.The Plant Journal,1999,18(1):111-119.［2］SUN J H，XU J D，QU W R,et al.Genome-wide analysis of R2R3 MYB transcription factors reveals their differential responses to drought stress and ABA treatment in desert poplar（Populus euphratica）［J］.Gene,2023,855:147124.［3］DUBOS C,STRACKE R,GROTEWOLD E,et al.MYB transcription factors in Arabidopsis［J］.Trends in Plant Science,2010,15(10):573-581.［4］MATSUI K,OSHIMA Y,MITSUDA N,et al.Buckwheat R2R3 MYB transcription factor FeMYBF1 regulates flavonol biosynthesis［J］.Plant Science,2018,274:466-475.［5］REDDY V A,WANG Q,DHAR N,et al.Spearmint R2R3-MYB transcription factor MsMYB negatively regulates monoterpene production and suppresses the expression of geranyl diphosphate synthase large subunit (MsGPPS.LSU)［J］.Plant Biotechnology Journal,2017,15(9):1105-1119.［6］ZHAO H X,YAO P F,ZHAO J L,et al.A novel R2R3-MYB transcription factor FtMYB22 negatively regulates salt and drought stress through ABA-dependent pathway［J］.International Journal of Molecular Sciences,2022,23(23):14549.［7］WANG S,WU H,CAO X,et al.Tartary buckwheat FtMYB30 transcription factor improves the salt/drought tolerance of transgenic Arabidopsis in an ABA-dependent manner［J］.Physiologia Plantarum,2022,174(5):e13781.［8］HSU P K,DUBEAUX G,TAKAHASHI Y,et al.Signaling mechanisms in abscisic acid-mediated stomatal closure［J］.The Plant Journal,2021,105(2):307-321.［9］董然,金鸽,林强,等.湖北百合LhrMYB1、LhrMYB12、LhrMYB15及LhrMYB16基因克隆及生物信息学分析［J］.北方园艺,2024(12):42-51.［10］唐彩群,雷秀锦,赵艳,等.香椿R2R3-MYB转录因子基因TsMYB1克隆、表达与功能分析［J］.北方园艺,2025(13):8-17.［11］田姗姗,黄诗宇,杨天为,等.高温干旱复合胁迫下铁皮石斛MYB基因家族鉴定及表达分析［J］.生物技术通报,2024,40(12):145-159.［12］赫卫,张慧.辣椒R2R3-MYB转录因子的全基因组鉴定及响应疫霉菌的表达模式分析［J］.农业生物技术学报,2021,29(4):641-655.［13］王林,陈晓雨,张文梦龙,等.大麦HvMYB2分子特性及响应干旱胁迫的功能分析［J］.作物学报,2025,51(4):873-887.［14］王丽平,王晓钰,傅竞也,等.玉米转录因子ZmMYB12提高植物抗旱性和低磷耐受性的功能鉴定［J］.作物学报,2024,50(1):76-88.［15］任明辉,张雨蓬,许涛,等.紫花苜蓿R2R3-MYB亚家族鉴定与干旱胁迫下的表达分析［J］.草地学报,2023,31(4):972-983.［16］李慧,张雨峰,李晓刚,等.基于转录组信息的杜梨盐胁迫相关MYB转录因子鉴定与分析［J］.农业生物技术学报,2024,32(1):132-146.［17］乔永刚,陈亮,王勇飞,等.药用植物金银花Dof基因家族的筛选及表达分析［J］.核农学报2020(9):1889-1897.［18］谭政委,郭水柱,苏小雨,等.全基因组水平金银花TCP基因家族的鉴定及表达模式分析［J］.中草药,2024,55(5):1665-1676.［19］李素君,文艺,闫文庆,等.金银花NBS-LRR基因家族的鉴定与表达分析［J/OL］.分子植物育种，（2026-01-02）［2026-01-12］.https://link.cnki.net/urlid/46.1068.s.20240304.1906.013.［20］杨教童,李鹏飞,肖巧巧.金银花WRKY基因家族的鉴定与功能分析［J］.西南大学学报(自然科学版),2023(7):87-96.［21］SEO P J,LEE S B,SUH M C,et al.The MYB96 transcription factor regulates cuticular wax biosynthesis under drought conditions in Arabidopsis［J］.The Plant Cell,2011,23(3):1138-1152.［22］LEE S B,KIM H U,SUH M C.MYB94 and MYB96 additively activate cuticular wax biosynthesis in Arabidopsis［J］.Plant & Cell Physiology,2016,57(11):2300-2311.［23］SEO P J,XIANG F,QIAO M,et al.The MYB96 transcription factor mediates abscisic acid signaling during drought stress response in Arabidopsis［J］.Plant Physiology,2009,151(1):275-289.［24］GIL L,LEE K,JOON S.The Arabidopsis MYB96 transcription factor plays a role in seed dormancy［J］.Plant Molecular Biology,2015,87(4):371-381.［25］LEE K,LEE H G,YOON S,et al.The Arabidopsis MYB96 transcription factor is a positive regulator of ABSCISIC ACID-INSENSITIVE4 in the control of seed germination［J］.Plant Physiology,2015,168(2):677-689.［26］GUO L,YANG H,ZHANG X,et al.Lipid transfer protein 3 as a target of MYB96 mediates freezing and drought stress in Arabidopsis［J］.Journal of Experimental Botany,2013,64(6):1755-1767.［27］GIL L,JOON S.The MYB96-HHP module integrates cold and abscisic acid signaling to activate the CBF-COR pathway in Arabidopsis［J］.The Plant Journal,2015,82(6):962-977.［28］JOON S,PARK C M.MYB96-mediated abscisic acid signals induce pathogen resistance response by promoting salicylic acid biosynthesis in Arabidopsis［J］.New Phytologist,2010,186(2):471-483.［29］PAYNE C T,ZHANG F,LLOYD A M.GL3 encodes a bHLH protein that regulates trichome development in Arabidopsis through interaction with GL1 and TTG1［J］.Genetics,2000,156(3):1349-1362.［30］SCHELLMANN S,SCHNITTGER A,KIRIK V,et al.TRIPTYCHON and CAPRICE mediate lateral inhibition during trichome and root hair patterning in Arabidopsis［J］.The EMBO Journal,2002,21(19):5036-5046.［31］ZIMMERMANN I M,HEIM M A,WEISSHAAR B,et al.Comprehensive identification of Arabidopsis thaliana MYB transcription factors interacting with R/B-like BHLH proteins［J］.The Plant Journal,2004,40(1):22-34.［32］DUARTE J M,CUI L,WALL P K,et al.Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis［J］.Molecular Biology and Evolution,2006,23(2):469-478.［33］PERAZZA D,VACHON G,HERZOG M.Gibberellins promote trichome formation by up-regulating GLABROUS1 in Arabidopsis［J］.Plant Physiology,1998,117(2):375-383.

Memo

Memo:
-
Last Update: 2026-03-16