|Table of Contents|

Optimization of Conditions for Liquid Fermentation of Ganoderma sinense Mycelium to Produce Triterpenoids

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2026年1
Page:
79-88
Research Field:
Publishing date:

Info

Title:
Optimization of Conditions for Liquid Fermentation of Ganoderma sinense Mycelium to Produce Triterpenoids
Author(s):
WEI JiaojunLUO YanglanLIAO YantingWANG CanqinWEI Shiyan
(Institute of Microbiology,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007)
Keywords:
G.sinensetriterpenoids contentfermentationresponse surface method
PACS:
S646
DOI:
10.11937/bfyy.20252324
Abstract:
Taking G.sinense strain SZ903 from Guangxi as the experimental material,the effects of liquid fermentation medium (nitrogen source,carbon source and pH) and fermentation conditions (temperature,rotational speed,incubation time,inoculation amount and bottled volume) on the content of triterpenoids were studied by single factor test combined with Plackett-Burman experiment,steepest ascent experiments and Box-Behnken response surface method,in order to provide reference for improving the yield of triterpenoids in the liquid fermentation of G.sinense in Guangxi.The results showed that the effects of maltose,yeast extract on G.sinense triterpenoids content were all extremely significant (P<0.01).The effects of the 3 factors on G.sinense triterpenoids content in fermentation culture were ranked as follows,yeast extract>maltose>fermentation temperature.The interaction between maltose and yeast extract,and fermentation temperature and yeast extract were significant influence on G.sinense triterpenoids content (P<0.05),the effects of interaction between maltose and fermentation temperature on G.sinense triterpenoids content had not significant (P>0.05).The optimum liquid fermentation medium and fermentation conditions were determined by verification test as follows:maltose 21.5 g·L-1,yeast extract 5.0 g·L-1,pH 5,fermentation temperature 26.9 ℃,incubation time 8 days,inoculum amount 8% and bottle volume 220 mL·(500mL)-1.Under these conditions,the G.sinense triterpenoids content was 64.23 μg·mL-1,which was 87.81% higher than that before optimization.

References:

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Last Update: 2026-01-16