|Table of Contents|

Identification of the SlPPDK Gene Sequence in Tomato and Its Expression in the Presence of Dark and Hypoxic Stress

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2026年4
Page:
1-8
Research Field:
Publishing date:

Info

Title:
Identification of the SlPPDK Gene Sequence in Tomato and Its Expression in the Presence of Dark and Hypoxic Stress
Author(s):
JIANG Zixuan; YU Youjian; REN Xinqi; ZHANG Silin; ZHU Zhujun
(College of Horticulture Science, Zhejiang A&F University/Innovation Center of Agricultural Effcient and Green Collaborative Production of Zhejiang Province/Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable，Ministry of Agriculture and Rural Affairs，Hangzhou，Zhejiang 311300)
Keywords:
tomato; pyruvate phosphate double kinase; gene cloning; expression analysis
PACS:
S641.2
DOI:
10.11937/bfyy.20252236
Abstract:
Taking tomato ‘Micro-Tom’ as the test material，the transcript structure，subcellular localization and expression pattern of SlPPDK gene under dark and hypoxia stress were studied by cDNA rapid amplification of cDNA ends (RACE) technology，subcellular localization vector construction and transient transformation，as well as qRT-PCR technology，the molecular characteristics of SlPPDK and its expression pattern under dark and hypoxia stress were clarified,in order to provide references for improving the yield and quality of tomatoes.The results showed that only one unique transcript of SlPPDK could be amplified in tomato by 5′ RACE，and the gene encoded a typical PPDK protein，which was widely distributed in the cytoplasm，cell membrane and nuclear membrane.The qRT-PCR analysis results showed that the expression level of SlPPDK in mature leaves of tomato gradually decreased with the extension of dark treatment time，and hypoxia treatment increased the expression level of SlPPDK gene in roots.There was only one transcript of tomato SlPPDK，encoding a typical cytoplasmic PPDK protein.Its function was regulated by light and participates in the defense response of tomato to root hypoxia.

References:

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Last Update: 2026-03-12