|Table of Contents|

Cloning and Expression Analysis of LiUFGT1Gene in Lagerstroemia indica

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2025年6
Page:
18-25
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of LiUFGT1Gene in Lagerstroemia indica
Author(s):
GAO Zilong12WANG Jinfeng2CHEN Zhuomei2LI Jiyuan3LIU Weixin3LI Yonghua1
(1.College of Landscape Architecture and Art,Henan Agricultural University,Zhengzhou,Henan 450002;2.Zhejiang Academy of Forestry,Hangzhou,Zhejiang 310023;3.Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Hangzhou,Zhejiang 311400)
Keywords:
Lagerstroemia indicaflower colorthe UDP glucose: flavonoid-3-O-glucosyltransferase (UFGT)expression analysisanthocyanin content
PACS:
S 685.99
DOI:
10.11937/bfyy.20243015
Abstract:
Taking red,pink,and white offspring from half sibling families of the Lagerstroemia indica variety ‘Jianminhong’ as test materials,the full-length LiUFGT1 gene was cloned and analyzed for bioinformatics and expression to explore the role of the UFGT gene in different flower colors of Lagerstroemia indica,and in order to provide reference for further research on the regulation mechanism of flower color and molecular breeding of L.indica.The results showed that the CDS region of the LiUFGT1 gene in Lagerstroemia indica was 1 203 bp,encoding a total of 400 amino acids and possessing a UDPGT conserved domain.The protein molecular weight was about 44.05 kD,with an isoelectric point (pI) of 5.051,and it belonged to an unstable hydrophilic protein.The phylogenetic tree analysis showed the closest genetic relationship with the pomegranate PgUFGT gene,with an amino acid similarity of 93%.The detection of anthocyanin content showed that the red flower offspring (R) had higher anthocyanin content during the peak and final flowering stages,while the pink flower offspring (P) gradually increased in anthocyanin content during the three flower development stages of bud,peak,and final flowering;the content of anthocyanins in the offspring of white flowers (W) was low,showing an overall downward trend.qRT-PCR analysis showed that the expression trend of LiUFGT1 at different flower development stages of R,P,and W was consistent with the changes in anthocyanin content.The results showed that the expression of LiUFGT1 gene was positively correlated with anthocyanins.

References:

[1]李春霞,乔中全,邓涪元,等.紫薇LiUFGT基因的克隆与生物信息学分析[J].湖南林业科技,2023,50(1):24-30.[2]林启芳,刘婷婷,刘洁茹,等.紫薇属与黄薇属植物花瓣类黄酮组成及含量分析[J].园艺学报,2021,48(10):1956-1968.[3]尚嘉琪,赵夏陆,原阳,等.地被菊‘紫重楼’花发育过程中花色苷含量和CmUFGT基因表达研究[J].植物生理学报,2017,53(2):191-197.[4]LIU W,FENG Y,YU S,et al.The flavonoid biosynthesis network in plants[J].International Journal of Molecular Sciences,2021,22(23):12824.[5]杨慧勤,王佳丽,李思蕤,等.茄科蔬菜花青素苷分子调控研究进展[J].生物工程学报,2022,38(5):1738-1752.[6]DONG N Q,LIN H X.Contribution of phenylpropanoid metabolism to plant development and plant-environment interactions[J].Journal of Integrative Plant Biology,2021,63(1):180-209.[7]刘洪峰.凤丹不同颜色花瓣的色素及花色形成相关基因表达分析[D].北京:北京林业大学,2015.[8]樊荣辉,黄敏玲,钟淮钦,等.香水文心兰花色相关基因的克隆及表达分析[J].福建农业学报,2012,27(7):707-710.[9]鞠志刚,张国仙,付军,等.艾纳香葡萄糖基转移酶基因的克隆与生物信息学分析[J].分子植物育种,2018,16(20):6657-6661.[10]WANG Z L,WANG S,KUANG Y,et al.A comprehensive review on phytochemistry,pharmacology,and flavonoid biosynthesis of Scutellaria baicalensis[J].Pharmaceutical Biology,2018,56(1):465-484.[11]刘恺媛,王茂良,辛海波,等.植物花青素合成与调控研究进展[J].中国农学通报,2021,37(14):41-51.[12]SU V,HSU B D.Cloning and expression of a putative cytochrome P450 gene that influences the colour of Phalaenopsis flowers[J].Biotechnology Letters,2003,25(22):1933-1939.[13]MORITA Y,HOSHINO A,KIKUCHI Y,et al.Japanese morning glory dusky mutants displaying reddish-brown or purplish-gray flowers are deficient in a novel glycosylation enzyme for anthocyanin biosynthesis,UDP-glucose:anthocyanidin 3-O-glucoside-2″-O-glucosyltransferase,due to 4-bp insertions in the gene[J].Plant Journal,2005,42(3):353-363.[14]张洪伟,梁毅,刘小义,等.洋葱UFGT基因的克隆和表达分析[J].核农学报,2015,29(9):1677-1686.[15]王迪,成冠超,祝利伟,等.欧李ChUFGT基因的克隆、生物信息学分析及亚细胞定位[J].分子植物育种,2023,21(3):782-788.[16]赵志常,张波,高爱平,等.芒果UFGT基因的克隆及表达分析[J].江苏农业科学,2016,44(5):31-34.[17]TSUDA T,YAMAGUCHI M,HONDA C,et al.Expression of anthocyanin biosynthesis genes in the skin of peach and nectarine fruit[J].Journal of the American Society for Horticultural Science,2004,129(6):857-862.[18]REN C H,GUO Y,XIE L F,et al.Identification of UDP-rhamnosyltransferases and UDP-galactosyltransferase involved in flavonol glycosylation in Morella rubra[J].Horticulture Research,2002.[19]陈敏,刘静,刘至柔,等.四季秋海棠BsUFGT基因克隆及功能验证[J].农业生物技术学报,2023,31(10):2087-2097.[20]姜丽娜,李纪元,范正琪,等.金花茶类黄酮糖基转移酶基因的克隆和功能初步研究[J].西北植物学报,2020,40(12):1989-1999.[21]招雪晴,沈雨,苑兆和.石榴UFGT基因克隆与表达特征[C].红河:第四届中国石榴博览会暨第十届全国石榴生产与科研研讨会,2022.[22]王璐,戴思兰,金雪花,等.植物花青素苷转运机制的研究进展[J].生物工程学报,2014,30(6):848-863.[23]袁美静,马誉,巫瑞,等.影响月季花瓣呈色的理化因子及花色苷组分分析[J].西北植物学报,2024,44(2):255-269.[24]廖望仪,沈芳杰,周洋丽,等.石蒜属种质资源花色多样性与花色苷相关性分析[J].植物生理学报,2021,57(10):2024-2032.[25]王俊青,张彦辉,易婉莹,等.基于转录组测序红秋葵果实转色期花青素生物合成相关基因研究[J].北方园艺,2022(14):9-16.[26]陈新娜,喻锌,张利英,等.菊花CmUFGT基因及其启动子的克隆与表达分析[J/OL].分子植物育种,(2022-09-28)[2024-03-01].https://kns.cnki.net/kcms2/article/abstract?v=Oa1N_PzK0nTZTVi9krhqPB2IAD5q42WQ7ltzem2y_g5yo6AHAetRTe OJt8jP3akeGqu_SE9ldLHf5s8ilD_SFO-aGf3z8ugHcOECeM61S Ckn5z978YZzRGoWj3u3enG2KT1mcm L1uPfpkzEi_8iDfsSOD qJSzCiV-eiKi66bDXTf49ajzk9bWuYWGu MErNXi&uniplatform=NZKPT&language=CHS?.[27]FUKUCHI-MIZUTANI M,OKUHARA H,FUKUI Y,et al.Biochemical and molecular characterization of a novel UDP-glucose:Anthocyanin 3′-O-glucosyltransferase,a key enzyme for blue anthocyanin biosynthesis,from gentian[J].Plant Physiology,2003,132(3):1652-1663.

Memo

Memo:
-
Last Update: 2025-04-03