|Table of Contents|

Discovery of Related Locus on Powdery Mildew Resistance Genes in Oriental Melon in Northeast Chinaand East China Based on GWAS

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年22
Page:
24-30
Research Field:
Publishing date:

Info

Title:
Discovery of Related Locus on Powdery Mildew Resistance Genes in Oriental Melon in Northeast Chinaand East China Based on GWAS
Author(s):
JIN RongrongCAO HongLIU SisiCHEN QiumingZHANG JinfengSHENG Hui
(Harbin Academy of Agricultural Sciences,Harbin,Heilongjiang 150029)
Keywords:
oriental melonpowdery mildewresequenceGWAS
PACS:
S 652
DOI:
10.11937/bfyy.20232500
Abstract:
The feasibility of genome-wide association study (GWAS) on the basic theory of powdery mildew resistance of thin-skinned muskmelon was studied by using resequencing method in 118 germplasm resources of thin-skinned muskmelon from Northeast and East China,in order to provide reference for enriching breeding materials for powdery mildew resistance of thin-skinned muskmelon.The results showed that a total of 4 460 133 SNP loci with high quality were obtained,and 61 excellent alleles related to powdery mildew were screened by the two models.Combined with functional annotation,DPH3 antibody protein,PPR protein,flavonol synthase,methylesterase and DMR6 oxygenase-l et.It was mainly involved in metabolic processes such as biosynthesis,amino acid metabolism,signaling pathway conduction,RNA degradation and DNA repair.The CsTLP gene on chromosome 7 played an important role in cucumber downy mildew resistance;EFM1 on chromosome 1 could be a good candidate gene for stress response mechanism research and stress resistance breeding.

References:

[1]崔浩楠.甜瓜白粉病抗性基因Cmpmr2F的精细定位及候选基因分析[D].哈尔滨:东北农业大学,2021.[2]莫龙飞.山东省甜瓜白粉病生理小种鉴定及抗性基因分子标记[D].长春;吉林农业大学,2021.[3]CUI H,FAN C,DING Z,WANG X,et al.CmPMRl and CmPMrs are responsible for resistance to powdery mildew caused by Podosphaera xanthii race 1 in melon[J].Theor Appl Genet,2022,135(4):1209-1222.[4]陈桂平,巩彩艳,范仁春,等.小麦TaEDS1基因的克隆和在白粉病抗性中的功能研究[C].哈尔滨:中国遗传学会第九次全国会员代表大会暨学术研讨会,2013.[5]裴冬丽.番茄白粉病抗性反应关键基因的鉴定及功能验证[D].郑州:河南农业大学,2011.[6]张孝廉.烟草白粉病抗性相关基因MLO的作用机理研究[D].贵阳:贵州大学,2022.[7]DESIRE V,KAREN K,OLIVER F.A mutated dph3 gene causes sensitivity of Schizosaccharomyces pombe cells to cytotoxic agents[J].Current Genetics Eukaryotes with Emphasis on Yeasts,Fungi,Mitochondria,Plastids,2017,63(6):1081-1091.[8]高媛媛.水稻PPR蛋白基因TCD34的克隆与功能分析[D].上海:上海师范大学,2020.[9]郭浩.拟南芥水杨酸羟化酶DLO2的功能研究及低水杨酸遗传学材料的创制[D].金华:浙江师范大学,2020.[10]STHANIE G,JULIE H,FLORENCE P,et al.AtPME3,a ubiquitous cell wall pectin methylesterase of Arabidopsis thaliana,alters the metabolism of cruciferin seed storage proteins during post-germinative growth of seedlings[J].Journal of Experimental Botany,2017,68(5):1083-1095.[11]YOSHIDA R,UMEZAWA T,MIZOGUCHI T,et al.The regulatory domain of SRK2E/OST1/SnRK2.6 interacts with ABI1 and integrates abscisic acid (ABA) and osmotic stress signals controlling stomatal closure in Arabidopsis[J].The Journal of Biological Chemistry,2006,281(8):5310-5318.[12]马凯恒,李子仪,李大培,等.核桃JrEFM1转录因子响应逆境及激素的表达模式[J].西南师范大学学报(自然科学版),2019,44(6):46-53.[13]BURDIAK P,RUSACZONEK A,WITON D,et al.Cysteine-rich receptor-like kinase CRK5 as a regulator of growth,development,and ultraviolet radiation responses in Arabidopsis thaliana[J].Journal of Experimental Botany,2015,66(11):3325-3337.[14]MOYRAND F,JANBON G.UGD1,encoding the Cryptococcus neoformans UDP-glucose dehydrogenase,is essential for growth at 37 degrees C and for capsule biosynthesis[J].Eukaryotic cell,2004,3(6):1601-1608.[15]王锦楠.生长调节因子GRF12a和GRF12b对杨树生长发育的作用及其机理研究[D].北京:中国林业科学研究院,2020.[16]CONN S J,CONN V,TYERMAN S D,et al.Magnesium transporters,MGT2/MRS2-1 and MGT3/MRS2-5,are important for magnesium partitioning within Arabidopsis thaliana mesophyll vacuoles[J].The New Phytologist,2011,190(3):583-594.[17]NAKAJIMA K,KAWAMURA T,HASHIMOTO T.Role of the SPIRAL1 gene family in anisotropic growth of Arabidopsis thaliana[J].Plant and Cell Physiology,2006,47(4):513-522.[18]BOCCA S N,ROJAS-BELTRAN J A,NOEL F,et al.Molecular cloning and characterization of the enzyme UDP-glucose:Protein transglucosylase from potato[J].Plant Physiology and Biochemistry,1999,37(11):809-819.[19]宗成文,房经贵,陶建敏,等.葡萄MADS-box家族基因保守片段的克隆与序列分析[J].果树学报,2008,25(1):27-32.[20]李玉红,毛双双,孟焕文.黄瓜-霜霉菌互作体系中CsPI、CsTLP和CsHIRP的初步研究[C].成都:中国园艺学会黄瓜分会第五届年会,2015.[21]梅丽艳,王喜庆,温玲,等.4%朵麦可水乳剂防治甜瓜白粉病研究[J].北方园艺,2015(23):132-134.

Memo

Memo:
-
Last Update: 2023-12-14