|Table of Contents|

Genetic Diversity Analysis of Natural Hybrid F1 Generation of Red-kernel Walnut Based on SRAP Molecular Labeling

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年20
Page:
28-33
Research Field:
Publishing date:

Info

Title:
Genetic Diversity Analysis of Natural Hybrid F1 Generation of Red-kernel Walnut Based on SRAP Molecular Labeling
Author(s):
SUN Yangyang1; WANG Xiaoying1; DONG Lijuan1; LYU Xinmin2; ZHANG Jiliang1
(1.Taishan Academy of Forestry Sciences，Tai′an，Shandong 271000；2.Forestry Farm of Culai Mountain of Tai′an City，Tai′an，Shandong 271000)
Keywords:
red-kernel walnut; hybrid F1 generation; SRAP; genetic diversity
PACS:
S 664.1
DOI:
10.11937/bfyy.20230641
Abstract:
Taking ‘Hill’‘Xiangling’‘Robert Livermore’ and 7 ‘Robert Livermore’ natural hybrid F1 superior plants as experimental materials, a total of 10 walnut varieties (superior plants) were analyzed for genetic diversity using SRAP molecular marker technology, in order to reveal the genetic diversity of the F1 generation of natural hybrid red walnut and provide reference for the breeding of new red walnut varieties.The results showed that a total of 120 bands were amplified from the 25 pairs of selected primers，and the polymorphism ratio was 80%.The average Nei′s genetic diversity index and Shannon information index were 0.271 0 and 0.406 5，indicating that the genetic diversity and genetic difference among the 10 walnut varieties (superior tree) were relatively rich.The genetic similarity coefficient of the 10 walnut varieties (superior tree) ranged from 0.591 7 to 0.808 3.Ten walnut varieties (superior tree) were divided into three categories when genetic similarity coefficient threshold of 0.704 were selected.‘Xiangling’ was classified into a separate class and had a distant relation with the other 9 walnut varieties (superior tree).Among the F1 generation of ‘Robert Livermore’，those with red seed coat color were clustered into one category，while those with yellow seed coat color were clustered into another category.

References:

［1］郗荣庭,张毅萍.中国果树志·核桃卷［M］.北京:中国林业出版社,1996.［2］李琳,张港港,张燕,等.红瓤核桃遗传和生物学特性观察及种仁品质分析［J］.河南农业大学学报,2022,56(4):611-621.［3］刘永辉,孟海军,杨莹,等.彩色核桃品种资源及开发利用［J］.果树资源学报,2021(2):90-94.［4］王根宪,董兆斌,王英宏.特异核桃品种及种质资源介绍［J］.果树资源学报,2021(1):90-92.［5］朱建朝,辛国,任志勇,等.2个品种红仁核桃的表型特征及营养成比较分析［J］.林业科技通讯,2022(9):50-55.［6］谭江平,曾秀丽,廖明安.西藏光核桃自然居群遗传多样性的SRAP分析［J］.草业学报,2012,21(6):213-220.［7］李慧峰,冉昆,王涛,等.利用SRAP标记构建山东省苹果资源指纹图谱［J］.沈阳农业大学学报,2020,51(4):470-475.［8］李亚兰,潘存德,陈虹.SRAP标记在五十份新疆人工栽培核桃种质资源的鉴定与应用［J］.北方园艺,2019(16):44-52.［9］燕佳文,武晓晓,唐艳,等.基于SRAP分子标记的11份柑橘种质材料遗传多样性分析［J］.分子植物育种,2021,19(2):664-671.［10］尚晓星,张安世,刘莹,等.玫瑰香系葡萄种质资源SRAP遗传多样性分析及指纹图谱构建［J］.分子植物育种,2020,18(6):1916-1922.［11］张坤,周源洁,李尧,等.基于SRAP和SCoT标记的猕猴桃种质遗传多样性分析及变异材料鉴定［J］.果树学报,2021,38(12):2059-2071.［12］胡福初,吴小波,陈哲,等.基于SRAP分子标记的特早熟荔枝种质资源遗传多样性分析［J］.热带作物学报,2021,42(4):920-926.［13］肖志娟,翟梅枝,许静,等.不同核桃品种的ISSR标记分析［J］.北方园艺,2013(4):103-107.［14］李国田,艾呈祥,张力思,等.核桃实生居群遗传多样性 ISSR分析［J］.植物遗传资源学报,2011,12(4):640-645.［15］王红霞,赵书岗,高仪,等.基于AFLP分子标记的核桃核心种质的构建［J］.中国农业科学,2013,46(23):4985-4995.［16］李国田,张美勇,相昆,等.基于ISSR标记的16个核桃品种遗传多样性分析及分子身份构建［J］.核农学报,2015,29(10):1884-1892.［17］张捷,李勤霞,张萍,等.基于SRAP分子标记新疆野核桃的遗传多样性分析［J］.植物遗传资源学报,2016,17(2):239-245.［18］张捷.新疆野核桃SRAP遗传多样性分析与核心种质的构建［D］.乌鲁木齐:新疆农业大学,2015.［19］敬丹,骆翔,陈利娜,等.基于SSR分子标记的78份核桃种质资源遗传多样性分析［J］.江西农业学报,2020,32(6):11-16.［20］李琳.红瓤核桃颜色形成及相关基因研究［D］.郑州:河南农业大学,2022.［21］刘永辉.红瓤核桃生物学特性及花青苷代谢相关MYB转录因子表达分析［D］.郑州:河南农业大学,2021.［22］赵伟,刘永辉,章露露,等.红瓤核桃〖STBX〗JrMYB4和JrMYB306〖STBZ〗基因的克隆及表达分析［J］.河南农业大学学报,2022,56(1):70-78.［23］LI Y Z,LUO X,WU C Y,et al.Comparative transcriptome analysis of genes involved in anthocyanin biosynthesis in red and green walnut (Juglans regia L.［J］.Molecules,2018,23(1):25-42.［24］PERSIC M,MAJA M P,HEIDI H,et al.Red walnut:Characterization of the phenolic profiles，activities and gene expression of selected enzymes related to the phenylpropanoid pathway in pellicle during walnut development［J］.Journal of Agricultural and Food Chemistry,2018,66(11):2742-2748.［25］ZHAO W,LIU Y H,LIN L,et al.Genome-wide identification and characterization of bhlh transcription factors related to anthocyanin biosynthesis in red walnut (Juglans regia L.)［J］.Frontiers in Genetics,2021(12):632509-632523.［26］WANG L,LIN L,ZHAO W,et al.Integrated metabolomic and transcriptomic analysis of the anthocyanin and proanthocyanidin regulatory networks in red walnut natural hybrid progeny leaves［J］.PeerJ,2022(10):14262-14285.［27］ZHAO W,WU W J,LIU Y H,et al.The TT2-type MYB transcription factor JrMYB12 positively regulates proanthocyanidin biosynthesis in red walnut［J］.Scientia Horticulturae,2023,307:111515-111525.［28］LI G,QUIROS C F.Sequenc-related amplified polymorphism (SRAP),a new marker system based on a simple PCR reaction：Its application to mapping and gene tagging in Brassica［J］.Theoretical and Applied Genetics,2001,103(2/3):455-461.［29］梁燕,韩传明,孙超,等.基于SSR标记的核桃种质资源遗传多样性与遗传结构分析［J］.北方园艺,2022(9):47-54.［30］肖志娟.不同核桃品种遗传多样性的ISSR和SSR分子标记分析［D］.杨凌:西北农林科技大学，2013［31］方贤胜,吴涛,肖良俊.基于广泛靶向代谢组学的浅黄色和紫色核桃内种皮成分差异分析［J］.食品科学,2021,42(12):215-221.［32］GRANAHAN G M,CHUCK L.‘Robert Livermore’,a persian walnut cultivar with a red seedcoat［J］.Hort Science,2004,39(7):1772.

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Last Update: 2023-11-30