|Table of Contents|

Bioinformatics and Expression Analysis of VyCIPK3Gene in Chinese Wild Grape Vitis yeshanesis cv.‘Yanshan’ Grape

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年15
Page:
33-40
Research Field:
Publishing date:

Info

Title:
Bioinformatics and Expression Analysis of VyCIPK3Gene in Chinese Wild Grape Vitis yeshanesis cv.‘Yanshan’ Grape
Author(s):
LIU Yanpu1; 2; XIAO Xue1; ZHAO Jianming1; ZHU Ziguo3
Xinxiang，Henan 453003；2.Henan Research Center for Horticultural Plant Resources Utilization and Germplasm Innovation Engineering，Xinxiang，Henan 453003；3.Shandong Grape Research Institute，Jinan，Shandong 250000)
Keywords:
‘Yanshan’ grape; VyCIPK3gene; plant growth regulators; abiotic stress; expression analysis
PACS:
-
DOI:
10.11937/bfyy.20224004
Abstract:
Taking Chinese wild ‘Yanshan’ grape (Vitis yeshanensis ‘Yanshan’) as the material,we cloned the VyCIPK3 gene,with GenBank accession number NM_001281283,and analyzed the gene expression of the VyCIPK3 gene in different tissues and organs of grapes,as well as in grapes under the induced by different plant growth regulators and abiotic stresses by using quantitative realtime PCR (qPCR),in order to provide reference for the further study on the molecular function of the CIPK gene in grapes and the exploration of molecular mechanisms of grapevine stress resistance.The results showed thatVyCIPK3gene expression was tissue specific and highly expressed in roots.TheVyCIPK3gene responded strongly to abscisic acid (ABA) and methyl jasmonate (MeJA) induction，and theVyCIPK3gene responded more strongly to salt stress among different adversity stresses.TheVyCIPK3gene played an important role in ‘Yanshan’ grape growth and development and protection against external adversity stress,and this study might help to understand the mechanism of stress resistance in wild grapes in China and provide a gene resource for grape stress resistance breeding.

References:

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Last Update: 2023-09-19