|Table of Contents|

Cloning and Expression Analysis of DcNAC48 Gene of Dendrobium catenatum Lindl.

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年10
Page:
93-100
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of DcNAC48 Gene of Dendrobium catenatum Lindl.
Author(s):
CHEN Yu1; XING Wenting1; 2; ZHANG Tingting3; LI Yuxin3; RAO Dandan1; ZHOU Yang3
(1.Hainan Academy of Forestry (Hainan Academy of Mangrove),Haikou,Hainan 571100;2.Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences (CATAS),Haikou,Hainan 571101;3.School of Horticulture,Hainan University/Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province,Haikou,Hainan 570228)
Keywords:
Dendrobium catenatum Lindl.; NAC; abiotic stress; gene expression
PACS:
-
DOI:
10.11937/bfyy.20222910
Abstract:
A NAC (NAM,ATAF1/2,CUC2) transcription factor gene DcNAC48 was cloned by PCR from Dendrobium catenatum Lindl..The characteristic structures of the gene were analyzed by bioinformatics and the expression patterns in different tissues and under different stresses were studied by quantitative real-time PCR (qRT-PCR),in order to lay a foundation for further study of the function of DcNAC48 and provide gene resources for improving the stress resistance of D.catenatum.The results showed that the total length of DcNAC48 gene was 909 bp,encoding 302 amino acids,and had a conserved NAC domain.There were various hormone responsive elements,stress responsive elements,growth and development responsive elements and light responsive elements in the gene promoter region.The expression patterns analysis showed that DcNAC48 had tissue specificity,and its expression level was the highest in the gynostemium.The gene expression patterns under different stress treatments indicated that DcNAC48 was upregulated by low temperature,salt stress,drought stress and high temperature stress,suggesting that DcNAC48 might be involved in the response of D.catenatum Lindl.to abiotic stress.

References:

[1]ZHU J K.Abiotic stress signaling and responses in plants[J].Cell,2016,67(2):313-324.[2]RABARA R C,TRIPATHI P,RUSHTON P J.The potential of transcription factor-based genetic engineering in improving crop tolerance to drought[J].OMICS:A Journal of Integrative Biology,2014,18(10):601-614.[3]AIDA M,ISHIDA T,FUKAKI H,et al.Genes involved in organ separation in Arabidopsis:Analysis of the cup-shaped cotyledon mutant[J].Plant Cell,1997（9）:841-857.[4]ZHU G,CHEN G,ZHU J,et al.Molecular characterization and expression profiling of NAC transcription factors in Brachypodium distachyon L[J].PLoS One,2015,10(10):e0139794.[5]NURUZZAMAN M,MANIMEKALAI R,SHARONI A M,et al.Genome-wide analysis of NAC transcription factor family in rice[J].Gene,2010,465(1/2):30-44.[6]TRISHLA V S,MARRIBOINA S,BOYIDI P,et al.GUS-reporter based analysis of the promoter activity of Gossypium hirsutum NAC transcription factor,GhNAC4 that is induced by phytohormones and environmental stresses[J].Plant Cell Tiss Org Cult,2020,141:643-654.[7]HAO Y J,WEI W,SONG Q X,et al.Soybean NAC transcription factors promote abiotic stress tolerance and lateral root formation in transgenic plants[J].Plant J,2011,68:302-313.[8]YARRA R,WEI W.The NAC-type transcription factor GmNAC20 improves cold,salinity tolerance,and lateral root formation in transgenic rice plants[J].Funct Integr Genomics,2021,21(3/4):473-487.[9]TRISHLA V S,KIRTI P B.Structure-function relationship of Gossypium hirsutum NAC transcription factor,GhNAC4 with regard to ABA and abiotic stress responses[J].Plant Sci,2021,302:110718.[10]刘雪娜,吴雪娇,刘顺航,等.铁皮石斛的药理作用及其保健食品研发进展[J].保鲜与加工,2021,21(10):144-150.[11]司灿.铁皮石斛对干旱胁迫的生理响应及DNA甲基化研究[D].厦门:华侨大学,2016.[12]NAGAHAGE I S P,SAKAMOTO S,NAGANO M,et al.An Arabidopsis NAC domain transcription factor,ATAF2,promotes age-dependent and dark-induced leaf senescence[J].Physiol Plant,2020,70(2):299-308.[13]REN Y,HUANG Z,JIANG H,et al.A heat stress responsive NAC transcription factor heterodimer plays key roles in rice grain filling[J].J Exp Bot,2021,72(8):2947-2964.[14]LI M,CHEN R,JIANG Q,et al.GmNAC06,a NAC domain transcription factor enhances salt stress tolerance in soybean[J].Plant Mol Biol,2021,105(3):333-345.[15]CHEN C J,CHEN H,ZHANG Y,et al.TBtools-an integrative toolkit developed for interactive analyses of big biological data[J].Mol Plant,2020,13(8):1194-1202.[16]张婷婷,李雨欣,张德遥,等.铁皮石斛蛋白磷酸酶PP2C家族基因鉴定及其表达分析[J].园艺学报,2021,48(12):2458-2470.[17]付亚娟,陈霞婷,乔洁,等.铁皮石斛亲环蛋白基因DoCyP的克隆及表达分析[J].园艺学报,2020,47(3):581-589.[18]OOKA H,SATOH K,DOI K,et al.Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana[J].DNA Res,2003,10(6):239-247.[19]NIE G,YANG Z,HE J,et al.Genome-wide investigation of the NAC transcription factor family in Miscanthus sinensis and expression analysis under various abiotic stress[J].Front Plant Sci,2021(12):766550.[20]LI L,HE Y,ZHANG Z,et al.OsNAC109 regulates senescence,growth and development by altering the expression of senescence-and phytohormone-associated genes in rice[J].Plant Mol Biol,2021,105(6):637-654.[21]DUAN M,ZHANG R,ZHU F,et al.A lipid-anchored NAC transcription factor is translocated into the nucleus and activates Glyoxalase I expression during drought stress[J].Plant Cell,2017,29(7):1748-1772.[22]WANG L,LI Z,LU M,et al.ThNAC13,a NAC transcription factor from Tamarix hispida,confers salt and osmotic stress tolerance to transgenic Tamarix and Arabidopsis[J].Front Plant Sci,2017(8):635.[23]KOU X,ZHOU J,WU C E,et al.The interplay between ABA/ethylene and NAC TFs in tomato fruit ripening:A review[J].Plant Mol Biol,2021,106(3):223-238.[24]AN J P,LI R,QU F J,et al.An apple NAC transcription factor negatively regulates cold tolerance via CBF-dependent pathway[J].J Plant Physiol,2018,221:74-80.[25]SHAN W,KUANG J F,LU W J,et al.Banana fruit NAC transcription factor MaNAC1 is a direct target of MaICE1 and involved in cold stress through interacting with MaCBF1[J].Plant Cell Environ,2014,37(9):2116-2127.[26]LI X L,YANG X,HU Y X,et al.A novel NAC transcription factor from Suaeda liaotungensis K.enhanced transgenic Arabidopsis drought,salt,and cold stress tolerance[J].Plant Cell Rep,2014,33(5):767-778.[27]LE HNANFF G,PROFIZI C,COURTEAUX B,et al.Grapevine NAC1 transcription factor as a convergent node in developmental processes,abiotic stresses,and necrotrophic/biotrophic pathogen tolerance[J].J Exp Bot,2013,64(16):4877-4893.[28]TAKASAKI H,MARUYAMA K,KIDOKORO S,et al.The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice[J].Mol Genet Genomics,2010,284(3):173-183.

Memo

Memo:
-
Last Update: 2023-07-06