|Table of Contents|

Optimization of Ultrasonic Assisted Extraction of Flavonoids From Bupleurum chinense by Response Surface Methodology and Its Antioxidant Activity

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年05
Page:
93-100
Research Field:
Publishing date:

Info

Title:
Optimization of Ultrasonic Assisted Extraction of Flavonoids From Bupleurum chinense by Response Surface Methodology and Its Antioxidant Activity
Author(s):
REN Qianqian; GAO Peng; MA Zhijing; ZHAO Min
(College of Landscape and Ecological Engineering，Hebei University of Engineering,Handan,Hebei 056038)
Keywords:
Bupleurum chinense; flavone; response surface methodology; ultrasonic assisted extraction; antioxidant
PACS:
-
DOI:
10.11937/bfyy.20222652
Abstract:
Taking Bupleurum chinense as the test material,extraction of total flavonoids from Bupleurum chinense by assisted with ultrasound was studied,the extraction process was optimized through single factor test and response surface test.The clear ability of total flavonoids to DPPH free radicals and ABTS+ free radicals and the content of total flavonoids in different parts of Bupleurum chinense Maxim were studied,in order to provide reference for the development and utilization of Bupleurum chinense.The results showed that the experimental model was very significant (P<0.000 1),and the goodness of fit between the predicted value of the model and the true value was good (R2=0.972 2).The optimum extraction conditions of ultrasonic assisted extraction of total flavonoids from Bupleurum chinense by response surface methodology were as follows,ultrasonic temperature 57 ℃,ultrasonic power 170 W,solid-liquid ratio 1∶156 mg?mL-1,ethanol concentration 74%.The yield of total flavonoids from Bupleurum chinense was 8.21%,which was in good agreement with the predicted value (8.17%).The order of total flavonoids content in different parts of Bupleurum chinense was leaf>stem>root.Total flavonoids from different parts had strong scavenging effects on DPPH free radicals and ABTS+ free radicals.

References:

[1]陈亚双,孙世伟.柴胡化学成分及药理作用研究进展[J].黑龙江医药,2014,27(3):630-633.[2]马文兵,曹端林,王建龙,等.北柴胡茎叶总黄酮提取工艺研究[J].中药材,2010,33(2):288-290.[3]宋林晓,邵娟娟.黄酮类化合物提取方法研究进展[J].粮食与油脂,2020,33(1):21-22.[4]李萌茹,周玉枝,杜冠华,等.中药黄酮类化合物抗衰老作用及其机制研究进展[J].药学学报,2019,54(8):1382-1391.[5]RENETA G,MAGDALENA K B,NIKOLAY D,et al.Flavonol 3-O-glycosides from three Algerian bupleurum species[J].Pharmacogn Mag,2015,11(41):14-23.[6]唐俊锋.柴胡茎叶性味功效探讨[J].光明中医,2016,31(12):1826-1828.[7]丛登立,孟宪兰,李鹏飞.柴胡茎叶总黄酮生物活性研究[J].人参研究,2021,33(6):32-34.[8]谭玲玲,蔡霞,胡正海.北柴胡各器官中总黄酮动态变化研究[J].中草药,2008,39(2):286-287.[9]谭玲玲,蔡霞,胡正海.狭叶柴胡中黄酮类化合物含量及其动态变化研究[J].南方农业学报,2012,43(2):223-226.[10]詹雪晶,蔡霈,蔡光明,等.小叶黑柴胡茎叶总黄酮对四氯化碳所致小鼠急性肝损伤的保护作用[J].中南药学,2011,9(4):241-243.[11]刘秀芳,李婷婷,蔡光明,等.小叶黑柴胡茎叶总黄酮体外抗氧化活性的研究[J].中南药学,2011,9(3):172-175.[12]唐志国,王斌,张腾霄.柴胡中黄酮类化合物提取条件的研究[J].黑龙江畜牧兽医,2016(3):121-123.[13]段英姿,客绍英.不同引种柴胡不同生长期地上部总黄酮含量比较研究[J].中国药学杂志,2015,50(5):395-398.[14]魏永生,王永宁,石玉平,等.分光光度法测定总黄酮含量的实验条件研究[J].青海大学学报(自然科学版),2003,21(3):61-63.[15]张子东,张圆圆,孟永斌,等.沙枣果总黄酮类化合物的富集研究[J].植物研究,2018,38(3):467-474.[16]张媛.MeJA和PAP1转录因子对丹参醋酸类成分积累的影响[D].西安:陕西师范大学,2011.[17]赵定华.山核桃内果皮黄酮提取、抗氧化及抑菌活性研究[D].杭州:浙江农林大学,2015.[18]成旭东.树莓叶黄酮提取的工艺优化对比研究[D].兰州:甘肃农业大学,2019.[19]高宁.老山芹黄酮的提取、活性分析及调理馅料的研制[D].哈尔滨:东北农业大学,2019.[20]黄丽华,陈刚.赪桐叶的总黄酮提取工艺优化[J].肇庆学院学报,2020,41(2):37-41.[21]刘杨,赵婧,梁莉,等.响应面优化蒲公英多酚超声波辅助乙醇提取工艺及其抗氧化性[J].食品工业科技,2017,38(2):287-292.[22]张桂芳,郭希娟,王瑞琦.响应面法优化超声波辅助萃取蒲公英多酚的研究[J].中国医院药学杂志,2017,37(5):421-426.[23]JI Y,GUO S,WANG B,et al.Extraction and determination of flavonoids in Carthamus tinctorius[J].Open Chemistry,2018,16(1):1129-1133.[24]程玉鹏,李弘琨,马爱萍,等.柴胡黄酮类抗氧化作用机制最新研究进展[J].化学工程师,2017(7):47-49.

Memo

Memo:
-
Last Update: 2023-04-11