Isolation and Identification of Endophytes and Actinomycetes From Underwood Planting Ganoderma lucidum
《北方园艺》[ISSN:1001-0009/CN:23-1247/S]
- Issue:
- 2023年03
- Page:
- 120-126
- Research Field:
- Publishing date:
Info
- Title:
- Isolation and Identification of Endophytes and Actinomycetes From Underwood Planting Ganoderma lucidum
- Author(s):
- LIU Yueqin; LUO Liyuan; LIU Xinrui; BAI Lu
- (College of Life Science,Yan′an University,Yan′an,Shaanxi 716000)
- Keywords:
- Ganoderma lucidum; endophytes; isolation; identification
- PACS:
- -
- DOI:
- 10.11937/bfyy.20222032
- Abstract:
- The underwood planting Ganderma lucidum was used as test material,the effect of different separation methods on the diversity of endophytic bacteria and actinomycetes of G.lucidum was studied by using 16S rDNA identification method,in order to find the strains or new species that produce active ingredients to promote the development of microbial medicine.The results showed that four strains belonging to one genus and one phylum of actinomycetes (LZ-F1,LZ-F2,LZ-F3,LZ-F4) and five strains belonging to four genera and two phyla of bacteria (LZ-B1,LZ-B2,LZ-B3,LZ-B4,LZ-B5) were isolated from healthy G.lucidum fruiting bodies.All four actinomycetes belong to Streptomyces,five strains belong to Peribacillius,Pseudomonas,Neorrhizobium and Bacillus resp