(1.Zhengzhou Institute of Agriculture and Forestry Sciences,Zhengzhou，Henan 450005；2.Hebi Agricultural Reclamation Agricultural Development Co.Ltd.,Hebi，Henan 458000)
The tissue separation process of dried mushroom of Phellinus igniarius was optimized through comparative experiments with Phellinus igniarius as the experimental material,in order to improve the separation success rate.The results showed that through the comparison of this experiment,the process of tissue isolation of dried mushroom Phellinus igniarius was optimized.It could be seen that the tissue block was soaked in water for 2-3 hours in advance,the tissue block was soaked in 75% alcohol for sterilization for 15-30 seconds,and antibiotics were added in PDA medium,which could greatly improve the success rate of separation,which could reach 80% or even up to 100%.