|Table of Contents|

Cloning and Expression Analysis of Flavanone 3-hydroxylase SlF3H-like Gene From Purple Tomato

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年17
Page:
26-33
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of Flavanone 3-hydroxylase SlF3H-like Gene From Purple Tomato
Author(s):
LIU Zhengjie1; 2; LIU Yuntong1; XU Shaozhong1; MAO Zichao1; 2; LIN Chun1; 2
(1.College of Agriculture and Biotechnology,Yunnan Agricultural University,Kunming，Yunnan 650201;2.Institute of Improvement and Utilization of Characteristic Resource Plants,Yunnan Agricultural University,Kunming，Yunnan 650201)
Keywords:
purple tomato; SlF3H-like gene; cloning; anthocyanins; expression analysis
PACS:
-
DOI:
10.11937/bfyy.20220892
Abstract:
The cherry purple tomato ‘Yanzi No.1’ was used as test material.RT-PCR,bioinformatics and qRT-PCR expression analysis were used to study the biological information of flavanone 3-hydroxylase gene SlF3H-like,in order to characterize the sequence characteristics and expression pattern of the gene.The results showed that the open reading frame (ORF) of SlF3H-like gene was 1 089 bp,encoding 362 amino acids.The random coil was found to occupy the majority of structural elements of SlF3H-like protein,followed by α-helix and extended chain.The F3H amino acid sequence of ‘Yanzi No.1’ was highly conserved from other species.SlF3H-like protein contained typical DIOX-N superfamily and 2OG-FeⅡ_Oxy domains.Phylogenetic analysis showed that ‘Yanzi No.1’ was closely related to ‘Pannali’ tomato,followed by potato.The anthocyanin content in leaves and pericarp of ‘Yanzi No.1’ was measured during its development.It was found that the anthocyanin content in pericarp gradually accumulated with the increase of fruit diameter,while the anthocyanin content in leaves increased first and then decreased sharply with the increase of leaf diameter.qRT-PCR was used to analyze the expression of SlF3H-like gene in the pericarp and leaves of ‘Yanzi No.1’.Results showed that the change trend of this gene expression was similar to that of anthocyanin content,indicating that SlF3H-like gene played an important role in anthocyanin accumulation of purple tomato.

References:

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Last Update: 2022-11-09