|Table of Contents|

Optimizing the Liquid Culture Medium of Phallus dongsun by Response Surface Methodology

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年16
Page:
106-112
Research Field:
Publishing date:

Info

Title:
Optimizing the Liquid Culture Medium of Phallus dongsun by Response Surface Methodology
Author(s):
HUANG Wanbing12LU Yingying12LIU Qingcheng12HUANG Xiaorun12GUI Yang12ZHU Guosheng12
(1.Guizhou Institute of Crop Germplasm Resources,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006;2.Guizhou Key Laboratory of Edible Fungi Breeding,Guiyang,Guizhou 550006)
Keywords:
Phallus dongsunresponse surface methodologyliquid spawnmedium
PACS:
-
DOI:
10.11937/bfyy.20220754
Abstract:
Taking the strain ‘PD1710141’ about Phallus dongsun as the test material,the response surface method were used to optimize the liquid culture medium,which involved Plackett-Burman design,path of steepest ascent and Box-Benhnken design,get the liquid culture medium with low cost and high yield,in order to provide reference for mass production of cultivated species about Phallus dongsun.The results showed that when the rotating speed was 150 r·min-1 and the liquid volume of shake flask was 150 mL,the composition of optimal liquid culture medium about Phallus dongsun were 10 g·L-1 glucose,1 g·L-1 potassium dihydrogen phosphate,2.5 g·L-1 peptone,0.8 g·L-1 magnesium sulfate,380 g·L-1 potato,1.5 g·L-1 yeast extract powder and 0.03 mg·L-1 vamin B1.Implementing this optimal medium,the fresh weight of mycelia was (4.300±0.038)g and was 97% of the predicted value.In conclusion,response surface method could be applied to the optimization of liquid culture medium and condition.

References:

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Last Update: 2022-10-12