|Table of Contents|

Cloning and Expression Analysis of CpCHS1 Gene of Cerasus pseudoceresus ‘Manaohong’

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年13
Page:
16-24
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of CpCHS1 Gene of Cerasus pseudoceresus ‘Manaohong’
Author(s):
LI Shuang; RAN Jiaxin; LI Xiaorong; HOU Qiandong; QIAO Guang
(Institute of Agricultural Bioengineering，Guizhou University/Key Laboratory of Mountain Plant Resources Protection and Germplasm Innovation，Ministry of Education，Guiyang,Guizhou 550025)
Keywords:
‘Manaohong’ cherry; chalcone synthase; gene cloning; expression analysis
PACS:
-
DOI:
10.11937/bfyy.20220028
Abstract:
Taking the root tissue of ‘Manaohong’ cherry as experimental materials，using PCR (RT-PCR) technology，the CpCHS1 gene was cloned，and its sequence was analyzed by bioinformatics and expression analysis.The expression of this gene in different tissues of the plant and its expression in response to stress were investigated,in order to provide reference for further analysis of the function of this gene in stress.The results showed that,the full length of ‘Manaohong’ cherry 〖STBX〗CpCHS1 〖STBZ〗was 1 227 bp，which encoded 408 amino acids.The relative molecular mass was 44.6 kD，the theoretical isoelectric point (pI) was 6.53；there was no transmembrane domain，and it was a stable hydrophilic protein；the protein signal peptide was predicted to be a non-secreted protein；the secondary structure included α-helix and no regular curling，etc.；multiple sequence alignments showed that CpCHS1 was closely related to Prunus persica，with a similarity of 100%.The results of fluorescence quantitative PCR showed that the expression of CpCHS1 in roots was the highest，followed by fruits and leaves.CpCHS1 expression changed significantly under drought，high salt，low temperature and high temperature.In conclusion ‘Manaohong’ cherry CpCHS1 had the highest expression level in the root system，and it responded significantly to adversity stress，which might be closely related to plant resistance.

References:

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Last Update: 2022-08-29