|Table of Contents|

Genetic Diversity and Genetic Structure Analysis of Walnut Germplasm Resources Based on SSR Markers

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年09
Page:
47-54
Research Field:
Publishing date:

Info

Title:
Genetic Diversity and Genetic Structure Analysis of Walnut Germplasm Resources Based on SSR Markers
Author(s):
LIANG Yan1; HAN Chuanming1; SUN Chao1; WANG Cuixiang1; LU Shidian2; MIN Xufeng3
(1.Shandong Academy of Forestry，Jinan,Shandong 250014;2.Forest Resources Monitoring and Protection Service Center of Shandong Haiyang,Haiyang,Shandong 265100;3.Zaozhuang Shanting District Natural Resources Bureau，Zaozhuang，Shandong 277500)
Keywords:
walnut; SSR molecular marker; genetic diversity; population structure; cluster relationship
PACS:
-
DOI:
10.11937/bfyy.20214296
Abstract:
Fifty eight walnut cultivars (superior trees) were used as test materials to evaluate the population genetic diversity,analyze the genetic structure characteristics,and constructe the NJ cluster dendrogram of them by fluorescent SSR molecular marker technology,in order to accurately understand the genetic background and pro-margin relationship of 58 walnut germplasm resources，provide reference for rational utilization of Julans regiagermplasm resources，conservation of genetic resources and development of reasonable breeding schemes and strategies.The results showed that,1)the 15 SSR primers screened out had high polymorphism，a total of 109 polymorphic alleles (Na) were amplified.The mean number of different alleles (Na) was 7.267，the mean number of effective alleles (Ne) was 3.069，observed heterozygosity (Ho) was 0.519，expected heterozygosity (He) was 0.653，polymorphism information content (PIC) was 0.603 5，and Shannon index (I) was 1.327.2)The genetic distance between 58 walnut cultivars ranged from 0.181 to 0.742，with a variation of 0.561.The proportion of genetic distance between 58 walnut cultivars 0.5 was 44.19%，and the proportion of genetic distance 0.6 was 13.5%.The 58 cultivars (superior trees) had broad genetic base，rich genetic information and high genetic diversity.3)Based on Nei genetic distance cluster analysis，58 walnut cultivars (superior trees) could be clustered into 3 groups.The clustering results were related to phenotypic traits and genetic origin，but not related to geographical location.4)In population genetic structure analysis，Q>0.6 accounted for 81.03%，and the proportion of gene mixed sources was high.

References:

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Last Update: 2022-06-14