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Molecular Cloning and Expression Analysis of Mn-SOD Gene From Auricularia heimuer

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年04
Page:
110-117
Research Field:
Publishing date:

Info

Title:
Molecular Cloning and Expression Analysis of Mn-SOD Gene From Auricularia heimuer
Author(s):
SUN Tingting1MA Yisha2LIU Ruipeng23SUN Jian2ZOU Li2
(1.College of Food Engineering,Harbin University,Harbin,Heilongjiang 150086;2.School of Forestry,Northeast Forestry University,Harbin,Heilongjiang 150040;3.School of Architectural Engineering and Emergency Management,Harbin Vocational and Technical College,Harbin,Heilongjiang 150036)
Keywords:
Auricularia heimuerMn-SOD genecloningprokaryotic expressionquantitative real-time PCR
PACS:
-
DOI:
10.11937/bfyy.20213549
Abstract:
Taking Auricularia heimuer DL202 as the test material,Mn-SOD gene was cloned by RT-PCR,and its bioinformatics analysis and expression analysis were studied,in order to lay the foundation for further study of its function in the stress resistance of A.heimuer.The results showed that Mn-SOD cDNA contained 609 bp and encoded 202 amino acids,named Ah-MnSOD.The amino acid sequence had Mn-SOD conserved domains and had no transmembrane structure and signal peptide.Subcellular localization showed that Ah-MnSOD was modified and synthesized in peroxisome.Phylogenetic analysis indicated that Ah-MnSOD had the closest relationship with Auricularia subglabra.In addition,the prokaryotic expression vector pET-32a-Ah-MnSOD was constructed and the target protein was successfully expressed by induction with IPTG.The results of qRT-PCR showed that Ah-MnSOD gene was all expressed during different stages of A.heimuer,and reached the highest level at the primordium stage.

References:

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Last Update: 2022-05-19