|Table of Contents|

Identification and Biological Characteristics of a Kiwifruit Leaf Spot Disease Pathogen

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年12
Page:
44-49
Research Field:
Publishing date:

Info

Title:
Identification and Biological Characteristics of a Kiwifruit Leaf Spot Disease Pathogen
Author(s):
SHI Jinqiao1; ZHANG Rongquan2; LONG Youhua1; 3; HU Anlong1; 3; MO Feixu1; LI Wenzhi1
(1.Institute of Crop Protection，Guizhou University，Guiyang,Guizhou 550025；2.Management Committee of Eastern Agricultural Industrial Park of Shuicheng County，Liupanshui，Guizhou 553000；3.Engineering and Technology Research Center of Kiwifruit，Guizhou University，Guiyang,Guizhou 550025)
Keywords:
kiwifruit; leaf spot; Lasiodiplodia theobromae; biological characteristics
PACS:
-
DOI:
10.11937/bfyy.20203880
Abstract:
Taking the kiwifruit leaf spot disease as test materials,the pathogens were separated and purified by tissue culture method and verified to be the pathogen through an artificial infection test.Pathogens were identified by morphological and molecular biological methods,the biological characteristic of different conditions pathogen were studied,in order to provide a reference for the investigation of the disease occurrence and the formulation of comprehensive preventive measures.The results showed that Lasiodiplodia theobromae was the pathogen of kiwifruit leaf spot disease.The mycelia could grow at 10-35 ℃,and 28 ℃ was the optimum temperature，optimum pH was 5，lighting conditions were continuous light,the best carbon source for mycelial rowth was sluble starch,the best nitrogen source was yast extract.

References:

[1]汤佳乐，黄春辉，吴寒，等.野生毛花猕猴桃果实表型性状及SSR遗传多样性分析[J].园艺学报，2014，41(6)：1198-1206.[2]赵金梅，高贵田，谷留杰，等.中华猕猴桃褐斑病病原鉴定及抑菌药剂筛选[J].中国农业科学，2013，46(23):4916-4925.[3]严平生，严英子.猕猴桃炭疽病的发生规律与综合防治[J].西北园艺(果树)，2014(1)：27-28.[4]罗禄怡，张晓燕.为害猕猴桃的两种叶斑病及防治[J].中国南方果树，2000(2)：40.[5]GUI Y L,GONG G S,YU X M,et al.First report of brown leaf spot on kiwifruit caused by Corynespora cassiicola in Sichuan,China[J].Plant Disease,2015,99(5):725.[6]KIKIHARA K,NAKASHIM C.Sooty spot of kiwifruit caused by Pseudocercospora actinidiae Deighton[J].Journal of General Plant Pathology,2008,74(2):185-187.[7]JEONG I H,LIM M T,KIM G H,et al.Incidences of leaf spots and blights on kiwifruit in Korea[J].The Plant Pathology Journal,2008,24(2):125-130.[8]潘慧，邓蕾，陈美艳，等.浙江省泰顺县猕猴桃周年病害调查及病原菌鉴定[J].江苏农业科学，2019，47(14)：104-111.[9]CROWS P W.Reassessment of the anamorph genera Botryodiplodia,Dothiorella and Fusicoccum[J].Sydowia,1999,51(2):167-175.[10]U〖JX-+0.8mm〗〖KG-*2/3〗＇RBEZTORRES J R,LEAVITT G M,GUERRERO J C,et al.Identification and pathogenicity of Lasiodiplodia theobromae and diplodia seriata,the causal agents of bot canker disease of grapevines in mexico[J].Plant Disease,2008,92(4):519-529.[11]孙荣华，路广亮，王章训.上海市鸡爪槭枝干溃疡病病原菌鉴定[J].植物保护学报，2019，46(4)：933-934.[12]周游.猕猴桃软腐病病原学研究[D].雅安：四川农业大学，2016.[13]廖旺姣，朱英芝，邹东霞，等.桉树枝枯病菌生物学特性[J].广西林业科学，2016，45(4)：369-372.[14]任亚峰，包兴涛，李冬雪，等.茶树叶斑病病原菌可可毛色二孢菌的鉴定[J].植物病理学报，2019，49(6)：857-861.[15]孙华，郭宁，石洁，等.海南玉米穗腐病病原菌分离鉴定及优势种的遗传多样性分析[J].植物病理学报，2017，47(5)：577-583.[16]YAN J Y,LI X H,KONG F F,et al.Occurrence of grapevine trunk disease caused by Botryosphaeria rhodina in China[J].Plant Disease,2011,95(2):129.[17]张玮，李兴红，郭飞飞，等.两种葡萄溃疡病菌双重PCR检测方法的建立与应用[J].植物保护学报，2017，44(4)：636-642.[18]张茜，郝媛媛，吴雪雍，等.一种由可可毛色二孢引起的春芋新病害[J].植物病理学报，2019，49(2)：276-280.[19]史国英，胡春锦，罗掉爱，等.毛葡萄穗轴褐腐病病原菌鉴定及其生物学特性[J].植物病理学报，2010，40(3)：242-249.[20]刘小玉，余凤玉，付登强，等.油茶叶斑病菌的分离、鉴定及生物学特性研究[J].中国热带农业，2019，86(1)：40-44.

Memo

Memo:
-
Last Update: 2021-09-26