|Table of Contents|

Establishment and Preliminary Application of PCR Detection Methods for Pantoea pleuroti

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年07
Page:
123-128
Research Field:
Publishing date:

Info

Title:
Establishment and Preliminary Application of PCR Detection Methods for Pantoea pleuroti
Author(s):
QIN Wentao; GU Tongtong; LIU Yu; SONG Zhongjuan; RONG Chengbo
(Institute of Plant and Environment Protection，Beijing Academy of Agriculture and Forestry Sciences/Beijing Engineering Research Center for Edible Mushroom/Key Laboratory of Urban Agriculture (North China) by Ministry of Agriculture and Rural Affairs,Beijing 100097)
Keywords:
Pleurotus eryngii; blight disease; Pantoea pleuroti; polymerase chain reaction; detection
PACS:
-
DOI:
10.11937/bfyy.20202726
Abstract:
Pantoea pleuroti，blight disease pathogen of Pleurotus eryngii was used as the test material，PCR primers were designed，synthesized and screened after analyzing the sequencing results of P.pleuroti genome，and the detection effect was studied，so as to establishefficient PCR detection system for P.pleuroti and provide molecular basis for scientific control of blight disease of P.eryngii.The results showed that the specificity of K3143f/R and K37f/R primers were strong，only when P.pleuroti genomic DNA was used as template，the PCR products showed a specific band of 660 bp and 666 bp，respectively，while the genomic DNA of 15 strains of Pantoea，three pathogens strains of common edible fungi disease and distilled water (negative control) were used as template,the PCR products showed no band.The detection systems based on these two primer pairs were of high sensitivity and were not interfered by the tissue fluid of P.eryngii，which could detect the lowest 3.6 pg?μ L-1 P.pleuroti genomic DNA.Furthermore，at least 100 cfu P.pleuroti could be detected when P.pleuroti had been inoculated onto the fruiting bodies of P.eryngii for 48 hours.In addition，primer K3143F/R was of higher efficiency than that of K37F/R.

References:

[1]张志鸿.杏鲍菇工厂化高效栽培关键技术研究[D].福州:福建农林大学，2013.[2]MA Y W，YIN Y，RONG C B，et al.Pantoea pleuroti sp.nov.，isolated from the fruiting bodies of Pleurotuseryngii[J].Current Microbiology，2016，72(2)：207-212.[3]RONG C B，YIN Y，MA Y W，et al.First report of Pantoea sp.induced bactertial blight of Pleurotu seryngii in China[J].Plant Disease，2015，97(1)：209-220.[4]钟宜科,邹大阳,赵彤，等.食源性致病菌核酸检测技术研究进展[J].食品研究与开发，2020，41(12)：218-224.[5]张宜文,赵海波,吴红,等.数字PCR在食品安全检测中的应用研究进展[J].分析测试学报，2020，39(5)：672-680.[6]MA Y W，RONG C B，WANG S X，et al.Development of a species-specific real-time PCR assay for diagnosing Pantoea beijingensis[J].Journal of Plant Pathology，2016，98(2)：365-368.[7]张欣媛，耿强.恶臭假单胞菌PCR检测方法的建立及应用[J].环境科学与技术，2018，41(11)：155-158.[8]李得铭,翟子翔,邓涛,等.番茄青枯菌分离与三重PCR体系建立[J].分子植物育种，2020，18(11)：3655-3661.[9]刘劼,焦彬彬,宋绍祎，等.丁香疫霉病菌PCR和实时荧光PCR检测[J].植物病理学报，2016，46(6)：730-738.[10]RONG C B，MA Y W，WANG S X，et al.Pantoea hericii sp.nov.，isolated from the fruiting bodies of Hericiumerinaceus[J].Current Microbiology，2016，72(6)：738-743.[11]WALTERSON A M，STAVRINIDES J.Pantoea：Insights into a highly versatile and diverse genus within the Enterobacteriaceae[J].FEMS Microbiol Rev，2015，39(6)：968-984.[12]XU F，LIU Y，MA Y W，et al.First report of Pantoea beijingensis induced soft rot disease of Pleurotus nebrodensis in China[J].Plant Disease，2014，98(7)：1000.

Memo

Memo:
-
Last Update: 2021-06-28