|Table of Contents|

Extraction Polysaccharides From Bletilla striata by Aqueous Two Phase System and Its Clearance to Nitrite

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年03
Page:
114-121
Research Field:
Publishing date:

Info

Title:
Extraction Polysaccharides From Bletilla striata by Aqueous Two Phase System and Its Clearance to Nitrite
Author(s):
WU Hongmin1; 2; GAO Qingqing1; 2; CHEN Geng1; 2; CHEN Junwen1; 2; SHA Bencai1; ZHAO Yan1; 2
(1.The Key Laboratory of Medicinal Plant Biology of Yunnan Province/National & Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwestern China,Yunnan Agricultural University,Kunming,Yunnan 650201;2.College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming,Yunnan 650201)
Keywords:
aqueous two phase system; Bletilla striata; polysaccharides; technology optimization; nitrite
PACS:
-
DOI:
10.11937/bfyy.20201469
Abstract:
Taking Bletilla striata as experimental material,the Ultrasonic-assisted PEG 6000/K2HPO4 were aqueous two phase system composed of polyethylene glycol 6000/dipotassium hydrogen phosphate,was applied to extract polysaccharides from Bletilla striata.The effects of solid-liquid ratio,extracted time,polysaccharide extract volume,PEG 6000 dosage and K2HPO4 dosage on polysaccharide extraction rate were analyzed by single factor and response surface methodology (BBD central composite).The results indicated that the polysaccharides extract volume and PEG 6000 dosage,the PEG 6000 and K2HPO4 dosage had significant interaction,while the polysaccharide extract volume and the amount of K2HPO4 had a lower interaction.The factor affected the extraction rate of polysaccharide were as follows,PEG 6000 dosage>polysaccharide extract volume>K2HPO4 dosage;the optimal process condition were 5.0 mL polysaccharide extract volume,K2HPO4 dosage was 1.0 g,and PEG 6000 dosage was 2.0 g.Three parallel tests were proceeded under optimal process conditions,the polysaccharide extraction rate was 72.81%,which was lower than the predicted value.The deviation was 0.94%,indicated that the process conditions were stable and feasible,and the results were reproducible.Bletilla striata polysaccharides had a specific effect on nitrite clearance,and the clearance rate increased along with the increase of polysaccharides concentration.This demonstrates the optimized extraction process of polysaccharides has great predictability，and this research can provide a suggestion for the efficient extraction of Bletilla striata polysaccharide.

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Last Update: 2021-06-04