|Table of Contents|

Rapid Propagation for Male Sterile Lines of Pigment Marigold

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年02
Page:
53-59
Research Field:
Publishing date:

Info

Title:
Rapid Propagation for Male Sterile Lines of Pigment Marigold
Author(s):
WEI Xiaomei; WU Lifang
(College of Biological Resource and Food Engineering，Qujing Normal University/Center for Yunnan Plateau Biological Resources Protection and Utilization，Qujing，Yunnan 655011)
Keywords:
pigment marigold; in vitro rapid propagation; male sterile lines
PACS:
-
DOI:
10.11937/bfyy.20200012
Abstract:
Pigment marigold was selected as research object.Leaves，stem with axillary buds and lateral buds of sterile plants from pigment marigold mother resources were used to research the effects of sterilization treatment，growth regulator combination and AgNO3 concentration on multiple shoots induction.The aim was to obtain the genetically stable male sterile plants of marigold，and provide basic research materials for cross breeding.The results showed that stem with axillary buds were the best explants.The best sterilization agent was 1 g?L-1 HgCl2 to sterilize 10-12 minutes.B1-3 was easy to induce multiple shoots，MS+1.0 mg?L-1 6-BA+0.5 mg?L-1 NAA+30 g?L-1 sucrose+7 g?L-1 agar was optimal induction medium，the induction rate for B1-3 was up to 93%.2.0 mg?L-1 AgNO3 was beneficial to proliferation of multiple shoots，and the proliferation rate of B1-3 was to 151.6%.After rooting culture，the survival rate of transplanting was over 95%.In vitro culture of marigold，the rate of cluster bud induction was different with different explants.Stem with axillary buds were the best explants.Different sterilization agents had different influenceson sterilization effect，1 g?L-1 HgCl2 of sterilization effect was the best.The combination of 6-BA and NAA was better than that of 6-BA and IAA for cluster buds induction.Low concentration AgNO3 could promote the induction of cluster buds，but high concentrations had opposite effect.

References:

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Last Update: 2021-05-10