|Table of Contents|

Study on Rapid Technique of Tissue Culture of Ranunculus asiaticus

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年01
Page:
48-53
Research Field:
Publishing date:

Info

Title:
Study on Rapid Technique of Tissue Culture of Ranunculus asiaticus
Author(s):
BAI Yanrong1; JIANG Yalian2; WANG Jinying1
(1.Kunming University，Kunming，Yunnan 650213；2.Flower Institute,Yunnan Academy of Agricultural Sciences，Kunming，Yunnan 650214)
Keywords:
Ranunculus asiaticus; multiplication culture; rooting culture; callus
PACS:
-
DOI:
10.11937/bfyy.20194837
Abstract:
The sterile seedlings of Ranunculus asiaticus were cultured on MS medium supplemented with 6-BA and NAA respectively to induce and proliferate in the primary generation of Ranunculus asiaticus，the experiment of Ranunculus asiaticus seedlings transplanting was carried out by subculture of rooting and different proportion of peat，vermiculite，perlite and river sand.The results showed that the best medium for bud induction was MS+1.5 mg?L-1 6-BA+0.2 mg?L-1 NAA，the induction rate was 100%，the medium for callus induction was MS+0.5 mg?L-1 6-BA+0.1 mg?L-1 NAA，the multiplication coefficient was 5.0.The subculture medium for rooting was 1/2MS+0.2 mg?L-1 NAA，the rooting rate was 89.9%，the best medium for transplanting was peat+river sand+vermiculite=6∶2∶2，survival rate was 100%.

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Last Update: 2021-04-08