|Table of Contents|

Construction of High Frequently Regeneration System From Leaves of Dwarfing Rootstock ‘Qingzhen D3’ of Pear

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年09
Page:
17-22
Research Field:
Publishing date:

Info

Title:
Construction of High Frequently Regeneration System From Leaves of Dwarfing Rootstock ‘Qingzhen D3’ of Pear
Author(s):
WANG Yue; YIN Jie; WANG Ran; YUAN Meitong; LI Dingli
(College of Horticulture,Qingdao Agricultural University/Qingdao Key Laboratory of Genetic Improvement and Breeding in Horticultural Plants,Qingdao,Shandong 266109)
Keywords:
pear; ‘Qingzhen D3’; leaves regeneration; tissue culture
PACS:
-
DOI:
10.11937/bfyy.20193750
Abstract:
High frequently regeneration system of leaves in vitro was established by using dwarfing rootstock ‘Qingzhen D3’ of pear as materials.The results showed that adventitious shoots of leaves in vitro could be induced by TDZ and IBA,and TDZ′s induction effect,which was proportional to its concentration,was better than that of IBA.The optimal culture medium of shoots regeneration was NN69+TDZ 3.0 mg?L-1+IBA 0.3 mg?L-1+agar 7 g?L-1+sugar 30 g?L-1,and 100.00% regeneration rate and 7.69 buds per leaf was achieved.And the results also revealed that adventitious roots could be induced by IBA and NAA,and induction effect of IBA was better than that of NAA.The optimal culture medium of roots induction was 1/2MS supplemented with IBA 0.2 mg?L-1,agar 7 g?L-1,and sugar 30 g?L-1,and 100% regeneration rate was achieved.This study laid a foundation for industrialization of seedling production of ‘Qingzhen D3’.

References:

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Last Update: 2020-07-08