|Table of Contents|

Cloning and Expression Analysis of Full-length cDNA Sequence of BnaWRKY75-like Gene in Brassica napus

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年09
Page:
1-8
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of Full-length cDNA Sequence of BnaWRKY75-like Gene in Brassica napus
Author(s):
YIN Mingzhi; HU Yan; YANG Shengjie; ZHANG Yu; YANG Lu
(College of Biology and Agricultural Science&Technology,Zunyi Normal University,Zunyi,Guizhou 563006)
Keywords:
Brassica napus; WRKY; transcription factors; gene cloning; expression analysis
PACS:
-
DOI:
10.11937/bfyy.20193740
Abstract:
WRKY superfamily was widely involved in many physiological processes and stress responses in plants.In this study,we used Brassica napus as experimental material,designed primers according to EST sequence obtained from transcriptome sequencing,and cloned the full-length cDNA sequence of WRKY transcription factor gene in Brassica napus by RT-PCR and RACE technology,in order to provide reference for the study of the function of the gene.The results showed that the coding sequence of BnaWRKY75-like gene was 444 bp,and the deduced amino acid sequence encoded 147 amino acid residues.There was a typical WRKY domain in the region of 67-124,including the core sequence of ‘WRKYGQK’.The similarity between the coding sequence of BnaWRKY75-like gene and that of Brassica rapa WRKY75 gene was 99.77%,and the amino acid sequence was the same.Semi quantitative RT-PCR analysis showed that the expression of BnaWRKY75-like gene increased after low temperature treatment,and the peak of expression appeared at 3 hours of treatment,which might play a role in the response to low temperature stress.

References:

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Last Update: 2020-07-08