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Analysis of Ultraviolet Mutagenesis Condition and Hybridization Effect of Pleurotus eryngii Strain

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年06
Page:
134-140
Research Field:
Publishing date:

Info

Title:
Analysis of Ultraviolet Mutagenesis Condition and Hybridization Effect of Pleurotus eryngii Strain
Author(s):
XU Yang; TANG Gaoxiang; LI Guanlin; ZHOU Jijiang; JI Ruiqing
(Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University,Changchun,Jilin 130118)
Keywords:
Pleurotus eryngii; UV mutagenesis; cross breeding
PACS:
-
DOI:
10.11937/bfyy.20193430
Abstract:
Pleurotus eryngii ‘Xingda’ was used as the test material,and the orthogonal experiments and the extremely poor methods were used to determine the mutagenesis time,mutagenesis distance and the spore concentration；the growth rate,fluorescence microscopy,colony characteristics observation,and the mushrooming state of the hybrid assemblage were used to evaluate the hybridization effect.The results showed that the optimal combination of UV mutation was 100 times the spore concentration,irradiation time 45 seconds,and vertical height 45 cm.Ten parental hybrids were formed by single colony hybridization after the mutagenesis,among them,the TX6 mycelium had the highest growth rate,which was significantly higher than that of the control(P<0.05);TX6,TX8,and TX10 produced mushrooms after the mushrooms were cultivated,and the mushroom bodies were ear-shaped,partially stipe-shaped,and no folds were seen on the back of the ear-pieces.These three hybrid combinations could be used as a new hybrid group to further study the mushrooming conditions.

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Last Update: 2020-05-26