|Table of Contents|

Gene Expression Related to Brown in Different Luffa Varieties

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年05
Page:
15-21
Research Field:
Publishing date:

Info

Title:
Gene Expression Related to Brown in Different Luffa Varieties
Author(s):
FENG Yingna1; 2; WANG Ran1; WANG Yuanhua1; 2; SU Xiaojun3; ZHANG Geng1; YAN Zhiming1; 2
(1.Jiangsu Polytechnic College of Agriculture and Forestry,Jurong,Jiangsu 212400；2.Jiangsu　Engineering and Technology Center for Modern Horticulture,Jurong,Jiangsu 212400；3.Jiangsu Academy of Agricultural Sciences,Nanjing,Jiangsu 210014)
Keywords:
Luffa; brown; activity of enzyme; gene expression
PACS:
-
DOI:
10.11937/bfyy.20192679
Abstract:
The different cultivars of Luffa were used as the research materials,to identificate postharvest Luffa fruit browning,activity of PPO,POD and relate gene express were analyzed.The results showed that ‘susi3’‘susi6’‘susi7’were brown resistant varieties,‘susi4’‘susi5’‘susi10’ were brown resistant varieties,while ‘susi8’ belonged to severe browning variety.According to the change of POD and PPO,the activity of POD of ‘susi8’ was 456.7 U·mg-1 prot,and that of resistant varieties ‘susi3’‘susi6’‘susi7’ were 250 U·mg-1.PPO activity of ‘susi8’ was 68.2 U·mg-1,and that of ‘susi3’ was the lowest,which was 25.2 U·mg-1 prot.According to the change of brown gene,the expression of LcPOD family gene in ‘susi8’ was higher than that of CK.The expression of [STBX]LcPOD3[STBZ] gene was 5.23,more than five times that of CK.There was no significant change in [STBX]LcPOD3[STBZ] gene in other Luffa varieties.The brown ‘susi8’ was significantly up-regulated in the LcPPO gene family.It showed that the expression of LcPPO gene family was enhanced during fruit of Luffa browning of ‘susi8’,and the expression of LcPPO gene family was translated into PPO enzyme to accelerate fruit browning.Compared with CK,the brown gene LCWRKY of Luffa was significantly up-regulated in ‘susi8’.It indicated that it was involved in postharvest browning of Luffa fruit.There was no significantly up-regulated in brown gene LCWRKY of others.

References:

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Last Update: 2020-04-05