|Table of Contents|

Isolation and Identification of Two Wild Strains of Lyophyllum spp.and Selection of Liquid Culture Medium

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年02
Page:
124-129
Research Field:
Publishing date:

Info

Title:
Isolation and Identification of Two Wild Strains of Lyophyllum spp.and Selection of Liquid Culture Medium
Author(s):
YUE Wansong1; GUO Xiang1; FENG Yunli1; MA Ming1; LIU Shaoxiong1; LUO Xiaokun2
(1.Kunming Edible Fungi Institute,All China Federation of Supply and Marketing Cooporatives,Kunming,Yunnan 650221;2.Acadamic Insitute,Yunnan Federation of Supply and Marketing Cooporation,Kunming,Yunnan 650221)
Keywords:
ITS sequence; Lyophyllum shimeji; Lyophyllum infumatum; liquid culture medium
PACS:
-
DOI:
10.11937/bfyy.20192616
Abstract:
Two wild strains,named C821-001 and C822-003,were isolated from two spieces of Lyophyllum spp.which were collected in Jizushan of Yunnan Province，then these two selected samples were identified by ITS sequence analysis.The liquid culture medium of C821-001 and C822-003 were selected by liquid shaking flask culture method with mycelial biomass as the main index,the liquid culture medium suitable for mycelial growth of two selected strains were selected from 5 kinds of culture medium which consisted of different materials.The results showed that C821-001 and C822-003 were respectively identified as Lyophyllum shimeji and Lyophyllum infumatum analyzed by phylogenetic relationship of ITS sequences.Both C821-001 and C822-003 grew best in formula 5,with the dry weight of mycelium 1.948 8 g and 1.128 8 g，respectively.So the formula 5 could be used as the preferred liquid culture medium for mycelium propagation of two selected strains.

References:

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Last Update: 2020-02-24