|Table of Contents|

Induction，Propagation and Conservation of Protocorm-like Bodys of Cymbidium hookerianum

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年10
Page:
71-77
Research Field:
Publishing date:

Info

Title:
Induction，Propagation and Conservation of Protocorm-like Bodys of Cymbidium hookerianum
Author(s):
FU Shuangbin1; 2; CHI Mengwei3; YANG Yanping1; YAO Lijuan1; ZHOU Zhuang1
(1.Institute of Subtropical Crops，Zhejiang Academy of Agricultural Sciences，Wenzhou，Zhejiang 325005;2.Fujian Ornamental Plant Germplasm Resources Innovation & Engineering Application Research Center,Fuzhou，Fujian 350002；3.College of Landscape，Fujian Agriculture and Forestry University，Fuzhou，Fujian 350002)
Keywords:
Cymbidium hookerianum; PLBs; propagation; encapsulation; conservation
PACS:
-
DOI:
10.11937/bfyy.20192474
Abstract:
In order to provide a new method for the propagation and germplasm resources conservation of Cymbidium hookerianum，the tissue culture seedlings of Cymbidium hookerianum were employed to evaluate the effects of adding different kinds and content of hormone on the induction of protocorm-like bodys (PLBs)，the effects of different media types，different hormone contents and activated carbon (AC) content on PLBs propagation，and the induced PLBs preserved via encapsulating by using single factor and orthogonal designs.The results showed that the addition of 3 mg·L-1 TDZ was optimal to the induction of PLBs；the optimal medium for PLBs propagation was 3 g·L-1 Hyponex 1+0.1 mg·L-1 NAA+3 mg·L-1 6-BA+1 g·L-1 AC；PLBs encapsulated by 3.5% sodium alginate solution and 0.1 mol·L-1 CaCl2 stored at 4 ℃ in the dark for 120 days，the germination rate reached 48%，conversion rate reached 44%.

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Last Update: 2020-07-25