|Table of Contents|

Isolation,Identification and Phylogenetic Tree Analysis of Endophytic Bacteria From Stipagrostis pennata

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年03
Page:
77-84
Research Field:
Publishing date:

Info

Title:
Isolation,Identification and Phylogenetic Tree Analysis of Endophytic Bacteria From Stipagrostis pennata
Author(s):
LI Xudong; MA Lei; ZHANG Tingting
(College of Life Sciences，Shihezi University，Shihezi，Xinjiang 832000)
Keywords:
Stipagrostis pennata; root; seed; endophytic bacteria; 16S rDNA
PACS:
-
DOI:
10.11937/bfyy.20191404
Abstract:
In this study,the endophytic bacteria Stipagrostis pennata isolated and purified from the tissues were studied by using traditional methods and culture-free methods.The morphological observation,physiological and biochemical identification of the isolated pure strains were carried out.The 16S rRNA gene sequence of the combined strain was identified in the RDP online database.The sequencing results were compared with the known sequences in GenBank,and the sequence of the most similar strains was searched for homology analysis,and then constructed phylogenetic tree by software MEGA 7.0 to determine the systematic taxonomic status of the strain.The results showed that 4 strains and 7 endophytic bacteria were isolated from the seeds and roots of the Stipagrostis pennata,and the bacteria were classified into 2 families,2 classes,2 orders,2 families and 2 genera,and the dominant species in seeds and roots.They were the genus Pantoea and Pseudomonas.The study showed that the endophytic bacterial community structure in the roots and seeds had rich population diversity,and the dominant degree of the flora was different.The research results provided favorable guidance and scientific basis for the accumulation of information on the endophytic resources of the Stipagrostis pennata and the subsequent development of the microbes.

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Last Update: 2020-02-26