|Table of Contents|

Breeding of Laccase High Yield Strain for Ganoderma applanatum by Compound Mutation

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年14
Page:
124-129
Research Field:
Publishing date:

Info

Title:
Breeding of Laccase High Yield Strain for Ganoderma applanatum by Compound Mutation
Author(s):
LIU XiaodanWANG JunlingWANG Xia
(School of Biological Engineering,Jilin Agricultural Science and Technology University,Jilin,Jilin 132101)
Keywords:
Ganoderma applanatumlaccaseUVdiethyl sulfatecompound mutation
PACS:
-
DOI:
10.11937/bfyy.20184621
Abstract:
The mycelium fragments of Ganoderma applanatum were used as test material,using ultraviolet ray and diethyl sulfate to induce mutagenesis step by step,in order to select and breed stable and high yield laccase strain,the mutants were primary screened in guaiacol-containing SPDA plates,and then the ability of the dominant strains to produce laccase was verified by triangular bottle fermentation experiment.The results showed that a mutant strain with high yield and genetically stable was obtained finally,laccase activity was 63.3% higher than that of the original strain,and the fermentation cycle was shortened by 1 day.

References:

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Last Update: 2019-08-12