|Table of Contents|

Optimization of Callus Induction Conditions and Anti-Browning of Red Cabbage

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年17
Page:
125-132
Research Field:
Publishing date:

Info

Title:
Optimization of Callus Induction Conditions and Anti-Browning of Red Cabbage
Author(s):
GUO Caizhen
(Department of Biology,Luliang University，Lyuliang,Shanxi 033000)
Keywords:
red cabbage; plant growth regulator; callus; response surface method; anti-browning agent
PACS:
-
DOI:
10.11937/bfyy.20183336
Abstract:
The callus was induced by the modified medium of MS+6-BA 0.5 mg?L-1+2,4-D 0.5 mg?L-1+NAA 0.8 mg?L-1 with the root segment，stem segment，leaf，flower and seed of red cabbage，and the best explant was screened according to its induction.By single factor and response surface test，the effects of three plant growth regulators 6-BA，2,4-D and NAA on callus induction of the best explants of red cabbage were studied.The effect of callus induction further was optimized the conditions for callus induction of red cabbage.The effects of different anti-browning agents on the callus induction of red cabbage were studied to screen out the best anti-browning agent which can effectively prevent or reduce the browning of callus and improve the callus induction rate and growth rate.The results showed that the stem was the best explant of callus induced by red cabbage,the induction rate of stem was 93.33%.The optimum concentration of 6-BA，2,4-D，NAA were 0.6 mol?L-1，0.8 mol?L-1，1.0 mol?L-1.The induction rate was 94.2%.It was suitable for the callus induced by the callus of the stem segment of the red cabbage:MS+6-BA 0.6 mg?L-1+2,4-D 0.8 mg?L-1+NAA 1.0 mg?L-1;0.2 g?L-1 AC was the best anti-browning agent induced by callus of red cabbage，the browning rate was 23.4% and the callus grows well.The best explants induced by callus of red cabbage and the optimization process were obtained in this experiment,it could provide a theoretical basis for the in vitro regeneration system of red cabbage.

References:

[1]杨红,操德群,朱江,等.响应曲面法优化红菜苔多酚提取工艺的研究[J].食品研究与开发,2013,34(8):5-8.[2]宋琼,任佐华,周湖,等.8种杀菌剂田间防治红菜苔根肿病的效果[J].作物研究,2015,29(4):415-418.[3]石云平,赵志国,唐凤鸾,等.白芨愈伤组织诱导、增殖与分化研究[J].中草药,2013,44(3):349-353.[4]马彦军,杨万鹏,杨坤,等.黄果枸杞愈伤组织诱导及快繁技术研究[J].干旱区资源与环境,2017,31(6):179-184.[5]周俊辉，周家容，曾浩森，等.园艺植物组织培养中的褐化现象及抗褐化研究进展[J].园艺学报，2000,27(z):481-486.[6]黄丹莹，江贵波.植物组织培养褐变产生的因素及对策[J].广西轻工业,2006(5):31-32.[7]王涛涛.红菜苔游离小孢子培养与植株再生[C].武汉:湖北省遗传学会第七次代表大会暨学术讨论会,2004.[8]李勇.红菜苔春化作用相关基因及其天然早花突变的分子机理研究[D].重庆:重庆大学,2009.[9]张建欣.紫斑牡丹愈伤组织诱导体系建立的研究[D].兰州:甘肃农业大学,2015.[10]杜雪玲,张振霞，刘萍,等.2,4-D和6-BA对多年生黑麦草愈伤组织诱导影响的研究[J].草原与草坪,2005(3):49-51.[11]周宜君,周生闯,刘玉,等.植物生长调节剂对植物愈伤组织的诱导与分化的影响[J].中央民族大学学报（自然科学版）,2007,16(1):23-28.[12]李书杰,张晓丽,李俊华,等.植物生长物质对山药微型块茎诱导形成的影响[J].北方园艺,2014(14):200-203.

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Last Update: 2019-09-24