|Table of Contents|

Identification of Pathogen Isolation and Pathogenicity Difference of ‘HongYang’ Kiwifruit Ripe Rot in Liupanshui City,GuiZhou Province

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年04
Page:
31-38
Research Field:
Publishing date:

Info

Title:
Identification of Pathogen Isolation and Pathogenicity Difference of ‘HongYang’ Kiwifruit Ripe Rot in Liupanshui City,GuiZhou Province
Author(s):
LEI JiqingWU WennengLIU YingLONG XiuqinLI WeijieWANG Rui
(Food and Pharmaceutical Engineering College,Guiyang University,Guiyang,Guizhou 550005)
Keywords:
kiwifruit(Actinidia spp.)ripe rotpathogenspathogenicity diversity
PACS:
-
DOI:
10.11937/bfyy.20182522
Abstract:
From 2016 to 2017,taking ripe rot kiwifruit collected from planting area in Guizhou Province Liupanshui city as raw material,and then the pathogens and pathogenicity differences were studied by pathogen isolation,pathogen identification,and pathogenicity identification in order to supply references for ripe rot prevention.The results showed that 23 pathogenic bacteria were isolated and obtained accession numbers MF498063-498065,MG775300-775302 and MG775283-775299,respectively;the isolated pathogens were Botryosphaeria sp.,Alternaria sp.,Phomopsis sp.,Neofusicoccum sp.and Diaporthe sp.,the detection rates of respective genus were 30.4%,30.4%,26.1%,8.7% and 4.4%;23 pathogenic bacteria experienced cluster analysis were divided into weak pathogenicity(26.09%),moderate pathogenicity(39.13%),strong pathogenicity(26.09%) and stronger pathogenicity(8.69%).

References:

[1]黄宏文,龚俊杰,王圣梅,等.猕猴桃属(Actinidia)植物的遗传多样性[J].生物多样性,2000,8(1):1-12.[2]王瑞玲.红阳猕猴桃采后病害生理及臭氧保鲜技术研究[D].雅安:四川农业大学,2010.[3]HAWTHORNE B T.Fungi associated with leaf spots and post-harvest fruit rots of kiwifruit (Actinidia chinensis) in New Zealand[J].New Zealand Journal of Botany,1982,20(2):143-150.[4]PENNYCOOK S R.Fungal fruit rots of Actinidia deliciosa (kiwifruit)[J].New Zealand Journal of Experimental Agriculture,1985,13 (4):289-299.[5]LATORRE B A,ALVAREZ C,RIBEIRO O K.Phytophthora root rot of kiwifruit in Chile[J].Plant Disease,1991,75(9):949.[6]LUONG L O.Phomopsis sp.associated with post-harvest fruit rot of kiwifruit in Italy[J].Journal of Plant Pathology,2011,93(1):205-209.[7]李黎,陈美艳,张鹏,等.猕猴桃软腐病的病原菌鉴定[J].植物保护学报,2016,43(3):527-528.[8]PENNYCOOK S R,SAMUELS G J.Botryosphaeria and Fusicoccum species associated with ripe fruit rot of Actinidia deliciosa (kiwifruit) in New Zealand[J].Mycotaxon,1986(24):445-458.[9]YOUNG J K.Postharvest fruit rots of kiwifruit (Actinidia deliciosa) in Korea[J].New Zealand Journal of Experimental Agriculture,2005,33(3):303-310.[10]YOU Z,GONG G S,CUI Y L,et al.Identification of Botryosphaeriaceae species causing kiwifruit rot in Sichuan Province,China[J].Plant Disease,2015,99(5):150204092345006.[11]李黎,陈美艳,张鹏,等.猕猴桃果实软腐病菌鉴定及抗性品种初步筛选[C]//中国植物病理学会2015年学术年会论文集.2015.[12]周游.猕猴桃软腐病病原学研究[D].雅安:四川农业大学,2016.[13]王小洁,李士谣,李亚巍,等.猕猴桃软腐病病原菌的分离鉴定及其防治药剂筛选[J].植物保护学报,2017,44(5):826-832.[14]邵力平,沈瑞祥,张素轩,等.真菌分类学[M].北京:中国林业出版社,1984.[15]SCHOCH C L,SEIFERT K A,HUHNDORF S,et al.Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi[J].Proceedings of the National Academy of Sciences of the United States of America,2012,109(16):6241-6246. [16]KUMAR S,STECHER G,TAMURA K.MEGA7:Molecular evolutionary genetics analysis version 7.0 for bigger datasets.[J].Molecular Biology & Evolution,2016,33(7):1870.[17]IVICA L,PEER B.Interactive tree of life (iTOL) v3:An online tool for the display and annotation of phylogenetic and other trees[J].Nucleic Acids Research,2016,44(Web Server issue):242-245.[18]程燕林,梁军,吕全,等.葡萄座腔菌科研究进展-鉴定,系统发育学和分子生态学[J].生态学报,2011,31(11):3197-3207.[19]李媛,石凌波,费诺亚,等.蓝莓间座壳茎溃疡病病原鉴定及生物学特性研究[J].植物保护,2017,43(1):89-94.[20]PHILIP R P.Development of resistance to picking wound entry Botrytis cinema storage rots in kiwifruit[J].New Zealand Journal of Experimental Agriculture,1994,22(4):387-392[21]ZHU Y,DONG W,MA J,et al.Characterization and virulence clustering analysis of extraintestinal pathogenic Escherichia coli isolated from swine in China[J].BMC Veterinary Research,2017,13(1):94.[22]黄征宇,李传道.杨树溃疡病菌致病力分化及杨树抗病性评价[J].南京林业大学学报(自然科学版),1987(1):25-34.[23]张星耀,赵嘉平,梁军,等.树木枝干溃疡病菌致病力分化研究[J].中国森林病虫,2008,27(1):1-4.[24]张艳杰,许换平,沈凤英,等.我国葡萄灰霉病菌形态型和致病力分化[J].农业生物技术学报,2017,25(11):1740-1755.

Memo

Memo:
-
Last Update: 2019-03-22