|Table of Contents|

RestorationofVitrifiedTest-tubePlantletsofLyciumbarbarum

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年03
Page:
138-143
Research Field:
Publishing date:

Info

Title:
RestorationofVitrifiedTest-tubePlantletsofLyciumbarbarum
Author(s):
LIUJing1; 2; SHIMing1; 2; QIAOGaixia1; 2
(1.NingxiaForestryInstituteCo.Ltd.,Yinchuan,Ningxia750004；2.StateKeyLaboratoryoftheSeedlingBioengineering,Yinchuan,Ningxia750004)
Keywords:
Keywords:Lyciumbarbarum; tissue-culturingseedling; vitrificationrecovery
PACS:
-
DOI:
10.11937/bfyy.20181421
Abstract:
Abstract:Theeffectsofdifferentbasicmedium,exogenousadditives,addingpolyacrylamide,differentcultureconditionsandshootsofdehydrationonvitrificationshootsrecoverybydifferentvitrifiedseedlingsofLyciumbarbarumasexplantsfortissueculturewerestudied.Themainpurposeofthestudywasexplorethemeasuresofwhichmakethevitrificationshootsrecovery.Theresultsshowedthat1/2MS+6-BA05mg?L-1+activatedcarbon05g?L-1wasthebestmediumforthemildvitreousseedlingsrecoveryofLyciumbarbarum.ItwassuitableforthemoderatevitrifiedseedlingsrecoveryofLyciumbarbarum1/4MS+6-BA05mg?L-1+activatedcarbon10g?L-1.Whenpolyacrylamide8g?L-1and16g?L-1wereadded,therecoveryrateofmildvitreousandmoderatevitrifiedseedlingsofLyciumbarbarumwasthehighest.ItnotonlycouldsignificantlyinhibitthegenerationofvitrficationofLyciumbarbarum,butalsohadsignificantreversaleffectwithvitrifiedtissue-culturingplantlets,whichgrewwellafterreverse.Afterdehydrationtreatment,thevitrifiedseedlingswereobviouslyrecovered.Therecoveryrateoflightvitrifiedseedlingsofdehydrated2dayscouldreach7761%.Withtheincreaseofthenumberofdaysofdehydration,therecoveryrateofmediumvitrifiedseedlingsofLyciumbarbarumwasgraduallyincreased.Whenthenumberofdehydrationdaysreached3days,thehighestrecoveryratecouldreach7124%.Enhancingmembranepermeabilityandlightintensitycouldalsoraisetherecoveryrateofvitrifiedseedlings.

References:

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Memo

Memo:
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Last Update: 2019-03-15