|Table of Contents|

Analysis on Genetic Diversity of Fifty-five Brassica campestris L.by  SSR Markers

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年12
Page:
7-12
Research Field:
Publishing date:

Info

Title:
Analysis on Genetic Diversity of Fifty-five Brassica campestris L.by  SSR Markers
Author(s):
XIE Jin; TENG Changcai; ZHAO Zhigang; YU Qinglan; ZHAO Hongchao
(Institute of Spring Rapeseed, Academy of Agriculture and Forestry Sciences, Qinghai University/The Qinghai Research Branch of The National Rapeseed Genetic Improvement Center/Scientific Observing and Experimental Station of Spring Rapeseed Ministry of Agriculture/Key Laboratory of Qinghai Province for Spring Rapeseed Genetic Improvement/Spring Rapeseed Research and Development Center of Qinghai Province, Xining, Qinghai 810016)
Keywords:
Brassica campestris L.; SSR; genetic diversity; germplasm resources
PACS:
-
DOI:
10.11937/bfyy.20180548
Abstract:
55 Brassica campestris L.inbred lines were used as experimental materials,the SSR molecular marker technique was used to study the genetic diversity of Brassica campestris L.in order to provide references for the selection of breeding combinations and germplasm innovation in breeding.The results showed that 44 pairs of stable polymorphic primers were screened out from 110 pairs of primers distributed on 10 chromosomes using SSR molecular markers,and 105 polymorphic loci were obtained.The number of bands amplified by each pair of primers was 2.4,and the average number of polymorphic sites per chromosome was 10.5.The genetic distance ranged from 0.016 9 to 0.754 4 with an average of 0.373 0.The differences among the tested materials were relatively large.55 clusters of materials were divided into 4 groups according to the cluster analysis,of which 31 were in the first category and 31 were in the second category.A total of 8 materials,Category III,Group 5,and Group IV,11 units.Among these materials,most of the same sources are clustered together,and a few individuals are distributed in different groups.This might be due to the variation of these materials under the influence of human and natural selection and cultivation environment.

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Last Update: 2018-07-03