Genetic Diversity Analysis of Colletotrichum spp.on Apple in North of China Based on ISSR Markers(PDF)
《北方园艺》[ISSN:1001-0009/CN:23-1247/S]
- Issue:
- 2018年13
- Page:
- 16-24
- Research Field:
- Publishing date:
Info
- Title:
- Genetic Diversity Analysis of Colletotrichum spp.on Apple in North of China Based on ISSR Markers
- Author(s):
- FU Dandan1; ZHANG Hongmei1; SUN Jianrui1; ZHAO Junfeng1; ZHANG Rong2; SUN Guangyu2
- (1.College of Food & Bioengineering,Henan University of Science and Technology/Key Laboratory of Microbial Resources Exploitation and Utilization,Luoyang,Henan 471023;2.College of Plant Protection,Northwest A & F University/State Key Laboratory of Crop Stress Biology for Arid Areas,Yangling,Shaanxi 712100)
- Keywords:
- apple bitter rot; Colletotrichum; ISSR molecular marker; genetic diversity
- PACS:
- -
- DOI:
- 10.11937/bfyy.20180371
- Abstract:
- To discuss the genetic diversity of pathogens associated with apple bitter rot at the molecular level,ISSR markers were applied to analyze the genetic diversity of 67 strains of Colletotrichum spp.causing bitter rot of apple in order to supply references for studying the epidemiology and control of apple bitter rot.The results showed that a total of 65 polymorphic bands were amplified with 10 screened primers,and the polymorphic loci percentage was 100.00%.Clustering analysis based on ISSR markers revealed that tested strains were clustered into seven groups at 0.76 genetic similarity coefficient by UPGMA.The results of genetic diversity of 67 strains from different geographical groups showed that Nei′s gene diversity index (H) and Shannon′s information index (I) were respectively greater than 0.2 and 0.3,which indicated that the genetic diversity all over China was considerably abundant.The results of population genetic structure of three geographical groups showed that Gst of the population from Bohai gulf area was the highest,indicating that the genetic diversity of this population was the most abundant and genetic differentiation among groups was bigger.The number of migrants per generation (Nm) of this group was less than 1,indicating that gene flow among groups was restrained.The Gst of the other population were lower but Nm were higher,indicating that genetic differentiation were lower and genetic variations existed within groups.There was significant gene flow within groups of old course of Yellow River and Northwestern Loess Plateau.The total Nm was 0.72,indicating that there was pathogen exchange among three geographical groups.
Last Update: 2018-07-24