Analysis on Genetic Diversity of Forty Different Wild Strains of Lentinus edodes

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Analysis on Genetic Diversity of Forty Different Wild Strains of Lentinus edodes

Author(s):: GUO Jinying; SONG Yanlong; LI Chao; CHEN Jian; ZHENG Suyue; ZHANG Qingqiao; (Landscape and Ecological Engineering，Hebei University of Engineering College，Handan，Hebei 056021)

Keywords:: Lentinus edodes; esterase isozyme; ISSR; genetic diversity; cluster analysis

Abstract:: Forty wild Lentinus edodes strains were used as experimental materials，the genetic diversity were identified and analyzed by esterase isoenzyme zymogram and ISSR molecular marker technique.The results showed that fifteen distinct bands were detected by the esterase isozyme zymogram.Cluster analysis results showed the forty wild Letinous edodes strains were divided into seven groups at genetic similarity of 71%.Seventy-five distinct bands which ranged from 0.2 kb and 4.0 kb were detected by gel electrophoresis.Cluster analysis results showed the forty wild Letinous edodes strains were divided into seven groups at genetic similarity of 63%.This study had laid a foundation for the research on germplasm resources of wild strains of Lentinus edodes and breed domestication.

[1]常广宁,吴春华.香菇新品种鉴定试验[J].中国食用,2015,34(1):16-19.

[2]MICALES J A,BONDE M R,PETERSON G L.The use of isozyme analysis in fungal taxonomy and genetics[J].Mycotaxon,1986,27(27):405-449.

[3]叶明,王和平,潘迎捷.利用酯酶同工酶技术检测香菇双单杂交后代[J].生物学杂志,1999,16(5):21-22.

[4]罗海燕,陈业渊.ISSR分子标记及其应用[J].安徽农学通报,2008,14(19):45-46.

[5]宋莹,刘俊杰,刘岩岩,等.辽宁省主栽香菇菌株ISSR遗传差异性分析[J].北方园艺,2015(5):82-85.

[6]胡能书,万贤国.同工酶技术及其应用[M].长沙:湖南科学技术出版社,1985.

[7]李辉平.应用ISSR标记对食用菌的遗传多样性分析[D].北京:中国农业科学院,2007.

Last Update: 2018-06-12