|Table of Contents|

Optimization of SSR-PCR Reaction System for Ziziphus acidojujuba

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年05
Page:
1-6
Research Field:
Publishing date:

Info

Title:
Optimization of SSR-PCR Reaction System for Ziziphus acidojujuba
Author(s):
CHANG Jing1; 2; LIU Qingbai1; LIU Mingguo1
1. College of Forestry, Shenyang Agricultural University, Shenyang ,Liaoning 110866；2.Liaoning Institute of Forest Management, Dandong, Liaoning 118002
Keywords:
Zizyphus acidojujuba; SSR-PCR reaction system;  optimization;  orthogonal design
PACS:
-
DOI:
10.11937/bfyy.20174451
Abstract:
Three Zizyphus acidojujuba clones LW1,LW2,LW23 were used as experimental materials, ‘Sanxing’ Ziziphus jujuba was as control, by orthogonal experimental design L16 (45) , the reaction system of SSR-PCR was established in order to provide the basis in the research method on SSR molecular markers of Zizyphus acidojujuba .The results showed that the optimal systems of SSR（20 μL）was DNA templates 100 ng, primer 0.5 μmol?L-1, Mg2+ 1.25 mol?L-1,Taq polymerase 1.5 U, dNTPs 0.3 mmol?L-1.By the reaction system, using primer JSSR250, DNA amplification of 53 Zizyphus acidojujuba clones and 8 Ziziphus jujuba varieties was carried out, which showed that the target strips were clear and the amplification products ranged from 140-180 bp, with 7 alleles. The optimal reaction systems could be used for the SSR molecular marker development of Zizyphus acidojujuba.

References:

[1] 刘孟军. 枣属植物分类学研究进展[J]. 园艺学报, 1999, 26(5): 302-308.

[2] 刘孟军, 王永蕙. 酸枣的优良品种和类型[J]. 中国果树, 1991(4):23-25.
[3] 李地军, 李萍. 酸枣的研究进展及开发前景[J]. 中国野生植物资源, 1999, 18(3): 15-19.
[4] 刘青柏, 刘明国, 纪连军, 等. 辽宁朝阳酸枣种质优特类型[J]. 中国果树, 2015 (6): 27-29.
[5] ROCHA R B, ABAD J I M, PIRES I E, et al. Fingerprint and genetic diversity analysis of Eucalyptus spp. genotypes using RAPD and SSR markers[J]. Scientia Forestalis/Forest Sciences, 2002(62):24-31.
[6] 王永路, 孙正海, 辛培尧,等. 基于园林植物的SSR标记开发及应用研究进展[J]. 现代园艺, 2015(7):4-6.
[7] 李娟, 伦涵宁, 李曼,等. 基于SSR标记的豫西枣品种与酸枣的群体遗传结构与基因流分析[J]. 科技视界, 2017(1):202-203.
[8] 张春梅, 殷晓, 李新岗, 等. 黄河沿岸酸枣遗传多样性研究[J]. 西北农林科技大学学报(自然科学版),2013,41(12):107-112,119.
[9] 刘青柏, 刘明国, 肖德平, 等. 辽西朝阳地区酸枣种质果实主要性状特征[J]. 林业科学, 2016, 52(4): 38-47.
[10] 肖京. 枣基因组 SSR 位点特征分析及引物开发[D]. 保定: 河北农业大学, 2014.
[11] 沈鹏, 董丽. 大苞萱草ISSR-PCR反应体系的建立与优化[J]. 生物技术通报, 2010(8):129-133.
[12] 许玉兰, 蔡年辉, 康向阳,等. SSR - PCR反应体系建立与优化的研究概述[J]. 生物技术, 2012, 22(2):73-76.
[13] 续九如, 黄智慧. 林业试验设计[M]. 北京: 中国林业出版社, 1998: 65-79.
[14] 彭程, 刘全兰, 王洪光,等. 山东鹅观草SSR-PCR反应体系的优化和验证[J]. 生物学杂志, 2012, 29(2):91-94.
[15] 韩骞, 王辉, 王进茂,等. 利用SSR引物通用性分析杨柳科树种遗传多样性[J]. 分子植物育种, 2009, 7(5):904-911.

Memo

Memo:
-
Last Update: 2018-03-26