Abstract

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Title:: Disease Symptom and Pathogen Identification of Lycium baraum Leaf Blight Disease

Author(s):: LIU Wei; ZHANG Qinde; WANG Duowen; HE Cai; CAO Hu; ZHANG Jun; (Wuwei Academy of Forestry Science，Wuwei，Gansu 733000)

Keywords:: Lycium baraum; leaf blight disease of L.baraum; Alternaria alternata; symptom; pathogen identification

Abstract:: In order to clarify the leaf blight disease symptom and pathogen species of Lycium baraum during 2015—2017,the leaf blight disease symptom was surveyed systematically in the representative L.bararum plantation where was located at Changcheng township，Wuwei，Gansu.The tissue separation and back-test were used to isolate the pathogen and determine its pathogenicity，and which was identified by morphological traits as well as sequence analysis of rDNA-ITS.The results showed that the samples from leaves and fruits of L.bararum were all able to be infected by the pathogen of leaf blight disease，and the symptom of indoor vaccination and field return was the same as in the filed.The pathogens of leaves and fruits could invade each other，and which showed the typical symptoms of leaf blight disease on leaves and fruits.The 158 strains were isolated from different samples，which were all identified as Alternaria alternata (Fr.) Keissler by morphological traits.By ITS sequence analysis and combined with morphological characteristics，it was further determined that the pathogen which caused leaf blight disease in different tissues of L.bararum was A.alternata.