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Title:: Cloning Analysis of Mitochondrial Intermediate Peptidase Encoding Gene and Its Application on A Mating-locus Identification in Agrocybe aegerita

Author(s):: YANG Weixian1; 2; ZHAO Yongchang2; CHEN Yuhui1; CHEN Weimin2; (1.College of Life Science，Southwest Forestry University，Kunming,Yunnan 650224；2.Institute of Biotechnology ＆ Germplasmic Resource，Yunnan Academy of Agricultural Sciences，Kunming,Yunnan 650221)

Keywords:: Agrocybe aegerita; mitochondrial intermediate peptidase encoding gene; A mating locus

Abstract:: Based on the genome sequence，mitochondrial intermediate peptidase encoding gene (mip) was cloned from two strains YSG and AC0007 using long-range PCR method，and the genetic characterization in Agrocybe aegerita strains population were also investigated to test the applicability of them as the molecular mark for polarity identification.The results showed that mip was co-segregated with A mating locus and therefore it could be used for the molecular for the cloning of the latter.Sequence analysis showed C terminal of MIP protein encoding region was highly conserved，and the special primer pairs were designed for this region could amplify the mip fragments in all tested strains.Additionally，the variable encoding regions in C terminal could be used as the targeting site for the A mating locus identification，and it provided an important basis for genetics and breeding of edible mushroom.