|Table of Contents|

Application of Liquid Chromatography Coupled With Mass Spectrometry forChemical Constituents Analysis of Panax ginseng Leaves

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年04
Page:
145-151
Research Field:
Publishing date:

Info

Title:
Application of Liquid Chromatography Coupled With Mass Spectrometry forChemical Constituents Analysis of Panax ginseng Leaves
Author(s):
HOU Wanchao; LI Sainan; GUO Lifei; HUANG Yu; WU Tong; LIU Chunming
The Central Laboratory,Changchun Normal University,Changchun,Jilin 130032
Keywords:
high performance liquid chromatography; pressurized liquid extraction; ginseng leaves; ginsenosides
PACS:
-
DOI:
10.11937/bfyy.20173212
Abstract:
The Panax ginseng leaves were employed as the target,the 50% ethanol aqueous was used to extraction the saponins from the Panax ginseng leaves via pressurized liquid extraction under 60 ℃ and 180 ℃.The analysis was processed by means of a reversed phase C18 column.The flow rate of the mobile phase was 0.5 mL?min-1；column temperature was 30 ℃.A photodiode array detector （PDA) and an electrospray ionization mass spectrometry （ESI-MS) were used to detect the chemical constituents at the same time.The results showed that the main chemical components from ginseng leaves were ginsenosides,the types and contents of the ginsenosides from the extracts under different temperatures were different.The contents of the ginsenosides Re,Rg1,Ra1,Rf,Rc,Rb1,Rb2,Rg2 and Rd from the 60 ℃ extract were relatively high,the ginsenosides 20R-Rg3 and 20S-Rg3 were also extracted.The chemical constituents from the 180 ℃ extract were ginsenosides Rg6,F4,Rk3,Rh4,20R-Rg3,20S-Rg3,20R-Rs3,20S-Rs3,Rk1,Rg5,Rs5 and Rs4.High temperature and high pressure could convert common ginsenosides to the ginsenosides 20R-Rg3,20S-Rg3 and other unsaturated ginsenosides including the ginsenosides Rk1,Rg5,Rg6,F4,Rs5 and Rs4.This study could provide the analytical methods for the ginsenosides from ginseng leaves.

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Last Update: 2018-02-27