|Table of Contents|

Screening and SRAP Analysis of Germplasm Resources for Resistance to CMV on Pepper

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年03
Page:
1-6
Research Field:
Publishing date:

Info

Title:
Screening and SRAP Analysis of Germplasm Resources for Resistance to CMV on Pepper
Author(s):
LI Ning1; YIN Yanxu1; WANG Fei1; YAO Minghua1; ZHAO Rongqiu2; LI Xueqiao3
1.Institute of Economic Crops,Hubei Academy of Agricultural Sciences,Wuhan,Hubei 430064;2.College of Horticulture,Yangtze University,Jingzhou,Hubei 434023;3.Institute of Vegetable Science,Hainan Academy of Agricultural Sciences,Haikou,Hainan 571100
Keywords:
pepper; cucumber mosaic virus(CMV); germplasm resources; SRAP analysis
PACS:
-
DOI:
10.11937/bfyy.20172842
Abstract:
The 283 pepper germplasm resources were used as materials,the germplasm resistance to CMV was screened by artificial inoculation method.Furthermore,genetic diversity of CMV-resistance pepper gerplasm was analyzed with SRAP in order to provide an important basis for pepper breeding with CMV-resistance.The results showed that one accession for resistance to CMV and 28 inbred lines for medium resistance to CMV were identified from 283 germplasm resources.The 29 lines were clustered into 2 groups by cluster analysis.The results provided direction for pepper breeding.

References:

[1]CARANTA C,PALLOIX A,LEFEBVRE V,et al.QTLs for a component of partial resistance to cucumber mosaic virus in pepper:Restriction of virus installation in host-cells[J].T A G,1997，94（3-4):431-438.

[2]CARANTA C,PFLIEGER S,LEFEBVRE V,et al.QTLs involved in the restriction of cucumber mosaic virus （CMV) long-distance movement in pepper[J].T A G,2002,104（4):586.
[3]KANG W H,HOANG N H,YANG H B,et al.Molecular mapping and characterization of a single dominant gene controlling CMV resistance in peppers （Capsicum annuum L.)[J].T A G,2010,120（8):1587-1596.
[4]GRUBE R C,ZHANG Y,MURPHY J F,et al.New source of resistance to cucumber mosaic virus in Capsicum frutescens[J].Plant Disease,2000,84（8):885-891.
[5]SUZUKI K,KURODA T,MIURA Y,et al.Screening and field trials of virus resistant sources in Capsicum spp.[J].Plant Disease,2007,87（7):779-783.
[6]毛爱军,耿三省,闫新跃,等.辣（甜)椒TMV、CMV及疫病抗性材料的鉴定[J].长江蔬菜,2004（2):48-50.
[7]王述彬,袁希汉,马艳青,等.中国辣椒优异种质资源评价[J].辣椒杂志,2002,17（4):244-247.
[8]赵娟,王立浩,云兴福,等.辣椒抗黄瓜花叶病毒遗传育种研究的回顾与展望[J].中国蔬菜,2009（18):1-7.
[9]张晓敏,张正海,毛胜利,等.我国辣椒种质资源对黄瓜花叶病毒的抗性及相关分析[J].中国蔬菜,2015（3):49-54.
[10]JING Z B,WANG X P,CHENG J M.Analysis of genetic diversity among Chinese wild Vitis species revealed with SSR and SRAP markers[J].G M R,2013,12（2):1962.
[11]UKOSKIT K,THOMPSON P G,WATSON C E J,et al.Identifying a randomly amplified polymorphic DNA （RAPD) marker linked to a gene for root-knot nematode resistance in sweetpotato[J].Journal of the American Society for Horticultural Science,1997,122（6):818-821.
[12]LI G,QUIROS C F.Sequence-related amplified polymorphism （SRAP),a new marker system based on a simple PCR reaction:Its application to mapping and gene tagging in Brassica[J].T A G,2001,103:455-461.
[13]刘君,李东,曾林,等.SRAP分子标记分析辣椒种质资源遗传多样性研究[J].中国调味品,2015（12):48-51.
[14]张兴桃,王林,高贵珍,等.安徽省辣椒品种遗传多样性的SRAP分析[J].分子植物育种,2016,14（9):2519-2525.
[15]许先松,刘志钦,林晓丹,等.基于形态及SRAP标记的辣椒资源遗传多样性及亲缘关系比较[J].福建农林大学学报（自然版),2011,40（1):48-53.
[16]YAO M,LI N,WANG F,et al.Genetic analysis and identification of QTLs for resistance to cucumber mosaic virus in chili pepper （Capsicum annuum L.)[J].Euphytica,2013,193（2):135-145.
[17]张书芬,傅廷栋,李媛媛,等.SRAP标记分析甘蓝型油菜多态性[J].华北农学报，2006,21（1):50-54.
[18]周坤华,方荣,陈学军,等.正交设计优化辣椒SRAP-PCR反应体系及引物筛选[J].江西农业大学学报,2010,32（3):595-600.
[19]孔秋生,李锡香,向长萍,等.栽培萝卜种质亲缘关系的AFLP分析[J].中国农业科学,2005,38（5):1017-1023.
[20]ROHLF F J.NTSYS-pc:Microcomputer programs for numerical taxonomy and multivariate analysis[J].American Statistician,1987,41（4):330.
[21]孔秋生.萝卜种质资源遗传多样性和亲缘关系的研究[D].武汉:华中农业大学,2003.

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Last Update: 2018-02-05