|Table of Contents|

Cryopreservation of Dormant Buds by Vitrification inAlmond Cultivars ‘Aifeng’

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年01
Page:
62-66
Research Field:
Publishing date:

Info

Title:
Cryopreservation of Dormant Buds by Vitrification inAlmond Cultivars ‘Aifeng’
Author(s):
JIANG Xi1; 2; ZHANG Qi1; ZHAO Shuzhen1; JIAO Peipei2; PANG Xin′an2; CHEN Jiali1
1.College of Plant Science，Tarim University，Alar，Xinjiang 843300；2.Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin,Xinjiang Production & Construction Corps，Alar，Xinjiang 843300
Keywords:
almond cultivars ‘Aifeng’; dormant bud; cryopreservation; vitrification
PACS:
-
DOI:
10.11937/bfyy.20172108
Abstract:
In order to provide a theoretical basis for the preservation of germplasm resources，the effects of sucrose pretreatment time，pretreatment time，loading time and PVS2 dehydration treatment time on the preservation of almond dormant buds were studied by using the method of vitrification and cryopreservation.The results showed that the dormant buds were removed from healthy plants，and the dormant buds were stripped to 0.6-1.5 mm （containing 1-2 leaf primordia) and cultured in MS containing 0.5 mol?L-1 sucrose pretreatment solution 1 day,at room temperature with the liquid loadingof dormant buds loaded 20 minutes，dehydrated 70 minutes under the condition of 0 ℃.Then added a small amount of fresh PVS2，quickly put into liquid nitrogen tank，saved the 15 days out，the dormant buds were thawed 2-3 minutes in a 40 ℃ celsius water bath，uninstalled 30 minutes using 1.2 mol?L-1 sucrose washing solution containing MS medium，after the aseptic paper sucked the washing liquid，it went to the recovery medium.Under the condition of 24 ℃ celsius，the dormant buds were cultured in dark 7 days，and then cultured in normal light.After 2 weeks，the survival rate was 61.01%.

References:

[1]李疆,胡芳名,李文胜,等.扁桃的栽培及研究概况[J]果树学报,2002,19（5):346-350.

[2]曾斌,李疆,罗淑萍,等.扁桃属植物种质资源鉴定的SSR分析研究[J].新疆农业科学,2009,46（1):18-22.
[3]梁艳霞,王占和,张志玲,等.我国扁桃发展现状、问题及发展前景[J].现代园艺,2015（12):9.
[4]WANG Q C,VALKONEN J P T.Ｃryotherapapy of shoot tips:Novel pathogeneradication method[J].Trends Plant Sci,2009,14:119-122.
[5]石茹,王芳,王舰.玻璃化法超低温保存技术及其研究进展[J].北方园艺,2011（24):231-235.
[6]傅翠娜,肖远辉,曾继吾,等.菠萝茎尖玻璃化法超低温保存及植株再生[J].西南农业学报,2011（5):1875-1878.
[7]NIINO T,SAKAI A,YAKUWA H,et al.Cryopreservation of in vitro-grown shoot tips of apple and pear by vitrification[J].Plant Cell,Tissue and Organ Culture,1992,28:261-266.
[8]石茹,王芳,王舰.马铃薯离体茎尖玻璃化法超低温保存[J].江苏农业学报,2013（2):272-277.
[9]朱文涛,周厚成,王子成.五叶草莓超低温保存及遗传稳定性分析[J].果树学报,2013（1):55-61.
[10]陈红,程秀枝,林田.刺梨茎尖的玻璃化超低温保存及植株再生[J].果树学报,2012（6):1069-1073,1154.
[11]艾鹏飞,罗正荣.柿休眠芽茎尖玻璃化法超低温保存及植株再生[J].中国农业科学,2003（5):553-556.
[12]晋玲,刘进,张延红,等.麻花秦艽休眠芽的玻璃化超低温保存及植株再生[J].中药材,2012（9):1374-1377.
[13]SAKAI A，KOBAYASHI S，OIYAMA I.Cryopreservation of nucellar cells of navel orange （Citrus sinensis Osb var.brasiliensis Tanaka) by vitrification[J].Plant Cell Rep,1990，9（1):30-33.
[14]HONG S R,YIN M H.A simple and efficient procedure for cryopreservation of embryogenic calli of medicinal plant Anemarrhena asphodeloides Bunge by vitrification[J].Plant Cell,Tissue Organ Culture,2012,109（2):287-296.
[15]张永卓,罗正荣.甜柿休眠芽茎尖包埋-玻璃化法超低温保存及植株再生[J].中国农业科学,2004（12):2019-2022.

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Last Update: 2018-01-12