|Table of Contents|

Polymorphism Analysis of TP-M13-SSR Primer for Prunus tenella Batsch.

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年02
Page:
46-51
Research Field:
Publishing date:

Info

Title:
Polymorphism Analysis of TP-M13-SSR Primer for Prunus tenella Batsch.
Author(s):
LIU Mengwen1; ZENG Bin1; 2; WANG Bo1; WANG Jianyou3
1.College of Forestry and Horticulture，Xinjiang Agricultural University，Urumqi，Xinjiang 830052；2.Research Center for Xinjiang Characteristic Fruit Tree，Xinjiang Agricultural University，Urumqi，Xinjiang 830052；3.Xinjiang Branch of Forestry Science,China Academy of Forestry Sciences,Urumqi,Xinjiang 830000
Keywords:
Prunus tenella Batsch.; TP-M13-SSR primer; capillary electrophoresis with fluorescent-labeled primer; characteristics of SSR loci
PACS:
-
DOI:
10.11937/bfyy.20171307
Abstract:
In this study,96 sample of leaves which were selected from 5 populations of Prunus tenella Batsch.were used as materials.The TP-M13-SSR technology and capillary fluorescent electrophoresis was used to analysis the polymorphism of it.By this way,it could provide the theoretical basis for the protection and conservation of this rare plant.The results showed that five types of repeat types and 23 627 sequences in the primers library.Dinucleotide was the largest,and pentanucleotide and hexanucleotide were the least.Using the 12 pairs core primers amplified with 96 samples,all of the No.of allele was 2.It was calculated that the number of effective alleles（Ne),observed heterozygosity（Ho),expected heterozygosity（He),fixation index（F),Shannon′s information index（I),the number of different alleles（Na),polymorphism information index（PIC),labeling index（MI),Nei′s deversity index,genetic similarity coefficient（Gst) and gene flow（Nm) ranged from 3 531 0-9 000 0,0-0 666 7,0 72-0 89,0 25-1 00,1 48-2 44,8-18,0 669 0-0 879 0,5 35-15 82,0 219 5-0 430 8,0 002 0-0 042 6,34 756 0-252 196 4,respectively.The polymorphism of primer PT-9 was best,and primer PT-1 was the worst.

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Last Update: 2018-01-26