|Table of Contents|

Disinfection and Browning Prevention for Mature Explant of Grapefruit(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年07
Page:
131-135
Research Field:
Publishing date:

Info

Title:
Disinfection and Browning Prevention for Mature Explant of Grapefruit
Author(s):
LIU YueYE WeiyanWANG YingtongLIU HuiminWANG Lianchun
(Key Laboratory of Biodiversity Conservation in Southwest State Forestry Administration,Southwest Forestry University,Kunming,Yunnan 650224)
Keywords:
Citrus paradisi Macfmature explantdinsinfectionbrowning
PACS:
-
DOI:
10.11937/bfyy.201707027
Abstract:
Stem segments of grapefruit were used as explants,the effects of different stem lengths,seedling varieties,disinfection methods and media types on disinfection result and browning prevention were studied.The results showed that 2 cm of stem section was the best disinfection effect.Difference of contamination and sprout rate existed among cultivars.‘Rio Red’ pollution rate was the lowest,the ‘Star Ruby’ was higher,but it had the highest germination rate.After treated by 75% ethanol for 20 seconds and followed by 5% hypochlorous sodium for 20 minutes,the materials were kept in culture room for 48 hours,then the material was treated by 5% hypochlorous sodium for 20 minutes again.This was the best way to disinfect with the minimum pollution rate 21.3%.With sodium hypochlorite secondary sterilization after vaccination in WPM+100 mg?L-1 cysteine medium,it could effectively control the degree of browning and get minimum pollution rate,only 15.6%,the highest germination rate was 83.1%.

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Last Update: 2017-04-19