|Table of Contents|

Vitality Determination and Storage Method for Pollen of Desmodium styracifolium(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年21
Page:
146-150
Research Field:
Publishing date:

Info

Title:
Vitality Determination and Storage Method for Pollen of Desmodium styracifolium
Author(s):
TANG Xiaomin; CHENG Xuanxuan; ZHANG Chunrong; PAN Haiyun; YANG Quan
School of Traditional Chinese Medicine,Guangdong Pharmaceutical University，Guangzhou，Guangdong 510006
Keywords:
Desmodium styracifolium; pollen vitality; storage method
PACS:
-
DOI:
10.11937/bfyy.20170601
Abstract:
The pollens of Desmodium styracifolium were employed as experimental material to study the effects of test methods and storage conditions on pollen vitality of D.styracifolium by staining method and culturation in vitro.The results showed that,the pollen vitality of D.styracifolium could be determined quickly by staining method,which was lower than that of culturation method in vitro.The optimal medium was 15% sucrose+0.01% H3BO3+0.09% Ca(NO3)2?4H2O+0.01% MgSO4?7H2O,in which the rate of germination was 96.16%,length of pollen tube was 541.72 μm;72-168 hours storage under 4 ℃ dry condition,it was good for the vitality maintenance of D.styracifolium pollens.The vitality of pollens dried by silicone was higher than the moist pollens.Culturation method in vitro was the most suitable method for D.styracifolium pollen viability determination,4 ℃ dry condition contributes was suitable for maintaining the pollen vitality of D.styracifolium.

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Last Update: 2017-11-14